caa a22 4500 477081231 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF02722980 doi (NATIONALLICENCE)springer-10.1007/BF02722980 Characterization of a new in vitro model for studies of reepithelialization in human partial thickness wounds [Elektronische Daten] [Katarina Jansson, Gunnar Kratz, Anders Haegerstrand] Summary: Reepithelialization of artificial partial thickness wounds made in biopsies of human skin was determined after 3, 5, or 7 d of incubation, submerged or elevated to the air-liquid interface. The biopsies were reepithelialized within 5-7 d, with a more complete epidermal healing in wounds exposed to air. Both types of wounds showed similar time-course in deposition of basement membrane components, as detected by immunofluorescence labeling. Laminin and collagen type VII were deposited underneath the migrating tips, whereas collagen type IV was detected after reepithelialization. Markers of terminal differentiation showed a pattern close to normal in the air-liquid incubated wounds after reepithelialization. Involucrin was detected in the suprabasal regions of the migrating epidermis and thereafter in the upper half of neo-epidermis in the air-liquid incubated wound. Filaggrin could not be detected in the submerged wounds at any time during healing, whereas wounds exposed to air showed a well-differentiated epidermis by Day 7. Tritiated thymidine-incorporation indicated proliferation of epidermal and dermal cells during reepithelialization and a maintained viability, as shown by cultivation of endothelial- and fibroblast-like cells obtained from the dermis 7 d after wounding. Reepithelialization in this humanin vitro model is supported by a matrix close to normal with the possibility of extracellular influences and cell-cell interactions and, in addition, the technique is simple and reproducible. Therefore, we suggest this model for studies of regeneration in culture and as a complement toin vivo studies on epidermal healing. Society for In Vitro Biology, 1996 reepithelialization nationallicence basement membrane nationallicence epidermal-dermal junction nationallicence terminal differentiation nationallicence Jansson Katarina Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut Kratz Gunnar Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut Haegerstrand Anders Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 534-540 1071-2690 32:9<534 1996 32 11626 https://doi.org/10.1007/BF02722980 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722980 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jansson Katarina Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 700 1- Kratz Gunnar Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 700 1- Haegerstrand Anders Department of Neuroscience, Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 534-540 1071-2690 32:9<534 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer