caa a22 4500 47708124X CHVBK 20180405111450.0 cr unu---uuuuu 170330e19961001xx s 000 0 eng 10.1007/BF02722981 doi (NATIONALLICENCE)springer-10.1007/BF02722981 Continuous cultures of macrophages derived from the 8-day epiblast of the pig [Elektronische Daten] [Neil Talbot, Mulumebet Worku, Max Paape, Patricia Grier, Caird Rexroad, Vernon Pursel] Summary: Secondary macrophage cell cultures were generated from the primary culture of epiblasts of 8-d-old pig blastocysts. The epiblast-derived macrophagelike (EDM) cells have a morphology and ameboid behavior that is typical of tissue histocytes. The cells reacted positively with monoclonal antibodies specific for pig granulocyte-macrophage lineage cells, and were not reactive with monoclonal antibodies specific for pig B and T lymphocytes. Marked phagocytic behavior and the formation of phagosomes were demonstrated following incubation with FITC-labeled bacteria. The EDM cells stained positively for nonspecific acid esterase that was not inhibited by sodium fluoride. DiI-acetylated-LDL was rapidly taken up by the cells. Transmission electron microscopy of the EDM cells showed phagolysosomes numerous cytoplasmic vacuoles, large, lobed nuclei, and numerous pseudopods or filopodia at the cell surface. Strong reactivity of the cells with anti-CD14 monoclonal antibody was observed. Further, cytotoxic activity was produced from the EDM cells after exposure to lipopolysaccharide in a concentration and time-dependent manner. The cultures could be maintained and expanded for several months on STO co-culture. Their derivation from the epiblast of the pig demonstrates the possibility of obtaining hemopoietic cell cultures from the preimplantation blastocysts of all mammals. Society for In Vitro Biology, 1996 culture nationallicence differentiation nationallicence epiblast nationallicence macrophage nationallicence pig nationallicence Talbot Neil Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut Worku Mulumebet Laboratory of Cellular Haematology, Division of Haematology, CBER, FDA, 8800 Rockville Pike, 20892, Bethesda, Maryland aut Paape Max Milk Secretion and Mastitis Laboratory, BARC-East, USDA, LPSI, ARS, Building 173, Room 103, 20705, Beltsville, Maryland aut Grier Patricia Growth Biology Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 120, 20705, Beltsville, Maryland aut Rexroad Caird Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut Pursel Vernon Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 541-549 1071-2690 32:9<541 1996 32 11626 https://doi.org/10.1007/BF02722981 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722981 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Talbot Neil Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut NATIONALLICENCE 700 1- Worku Mulumebet Laboratory of Cellular Haematology, Division of Haematology, CBER, FDA, 8800 Rockville Pike, 20892, Bethesda, Maryland aut NATIONALLICENCE 700 1- Paape Max Milk Secretion and Mastitis Laboratory, BARC-East, USDA, LPSI, ARS, Building 173, Room 103, 20705, Beltsville, Maryland aut NATIONALLICENCE 700 1- Grier Patricia Growth Biology Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 120, 20705, Beltsville, Maryland aut NATIONALLICENCE 700 1- Rexroad Caird Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut NATIONALLICENCE 700 1- Pursel Vernon Gene Evaluation and Mapping Laboratory, BARC-East, USDA, LPSI, ARS, Building 200, Room 13, 20705, Beltsville, Maryland aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/9(1996-10-01), 541-549 1071-2690 32:9<541 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer