caa a22 4500 477081258 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02723004 doi (NATIONALLICENCE)springer-10.1007/BF02723004 The interaction of human fetal neurons and epidermal cells in vitro [Elektronische Daten] [Mark Penfold, Patricia Armati, Zorka Mikloska, Anthony Cunningham] Summary: the interaction of autologous human fetal neurons with epidermal cells was studied by culturing fetal dorsal root ganglia (DRG) in the center of a dual chamber system with epidermal explants in the outer chamber. The two chambers were separated by two concentric stainless steel annular rings adherent to the substratum by silicon grease and agarose. Axons from the DRG penetrated the agarose barrier, growing into the exterior chamber by 10 din vitro (DIV) and extended past sparse peripheral fibroblasts to interact specifically with epidermal cells by 12 to 16 DIV. Scanning electron microscopy (SEM) showed single or multiple neuronal fascicles terminating on epidermal cells with spatular, veillike or bulbous axon termini. Transmission electron microscopy (TEM) showed fine axonal termini between epidermal cells, separated by an intercellular gap. The specificity of axonal targeting for epidermal cells rather than fibroblasts was also demonstrated by infecting the DRG with Herpes simplex virus type-1 (HSV-1). Specific anterograde transport of HSV-1 along axons to keratin-expressing epidermal cells was demonstrated by immunofluorescence and immunoperoxidase staining using monoclonal antibodies to viral glycoprotein D. This model allows the study of the mechanism of the specific interactions between neurons and epidermal cells analogous to those in fetal development and after cutaneous nerve regeneration. Society for In Vitro Biology, 1996 Neurons nationallicence epidermal cells nationallicence dorsal root ganglia nationallicence axon termini nationallicence Herpes simplex virus ultra-structure nationallicence Penfold Mark Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut Armati Patricia School of Biological Sciences, University of Sydney, 2001, Sydney, Australia aut Mikloska Zorka Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut Cunningham Anthony Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 420-426 1071-2690 32:7<420 1996 32 11626 https://doi.org/10.1007/BF02723004 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723004 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Penfold Mark Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut NATIONALLICENCE 700 1- Armati Patricia School of Biological Sciences, University of Sydney, 2001, Sydney, Australia aut NATIONALLICENCE 700 1- Mikloska Zorka Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut NATIONALLICENCE 700 1- Cunningham Anthony Virology Department, Centre for Infectious Diseases and Microbiology, University of Sydney, ICPMR, Westmead Hospital, Westmead, 2145, Sydney, Australia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 420-426 1071-2690 32:7<420 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer