caa a22 4500 477081290 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02723002 doi (NATIONALLICENCE)springer-10.1007/BF02723002 Isolation and characterization of microvascular endothelial cells from chicken fat pads [Elektronische Daten] [Waleed Twal, Roland Leach] Summary: Microvascular endothelial cells from abdominal fat pads of 6-wk-old broiler chickens were isolated to provide anin vitro system to study their physiological functions. The isolation procedure produced clumps of 10-30 cells, which attached to culture vessels in 4 h and attained confluency in 2 wk. At confluency, cells had a cobblestone appearance but were not contact inhibited and detached from the bottom of the culture vessel 2 wk after reaching confluency. The cells internalized acetylated low density lipoproteins, a characteristic of endothelial cells. This property was used to obtain pure endothelial cell cultures using the cell sorter. When cultured over Matrigel, a reconstituted matrix, the cells aligned themselves into chordlike structures and formed branching microvessels. Cells plated on type I collagen-coated culture flasks occasionally formed chordlike structures and proliferated at a faster rate than cells plated on Matrigel. Cells cultured on laminin-coated plates were slender and had long cytoplasmic extensions however, cells cultured on uncoated plastic had fibroblastic morphology. These properties are similar to those described for microvessel endothelial cells isolated from tissues of other species. Society for In Vitro Biology, 1996 endothelial cells nationallicence chickens nationallicence cell isolation nationallicence Matrigel nationallicence Collagen I nationallicence laminin nationallicence Twal Waleed Department of Poultry Science, The Pennsylvania State University, 213 Henning Building, 16802, University Park, Pennsylvania aut Leach Roland Department of Poultry Science, The Pennsylvania State University, 213 Henning Building, 16802, University Park, Pennsylvania aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 403-408 1071-2690 32:7<403 1996 32 11626 https://doi.org/10.1007/BF02723002 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723002 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Twal Waleed Department of Poultry Science, The Pennsylvania State University, 213 Henning Building, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Leach Roland Department of Poultry Science, The Pennsylvania State University, 213 Henning Building, 16802, University Park, Pennsylvania aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 403-408 1071-2690 32:7<403 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer