caa a22 4500 477081355 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02723008 doi (NATIONALLICENCE)springer-10.1007/BF02723008 Mycobacterium tuberculosis heart shock protein 10 increases both proliferation and death in mouse P19 teratocarcinoma cells [Elektronische Daten] [Grazia Galli, Pietro Ghezzi, Paolo Mascagni, Fabrizio Marcucci, Maddalena Fratelli] Summary: Exogenously addedMycobacterium tuberculosis Hsp10, either synthetic or recombinant, but not other related heat shock proteins (GroES fromEscherichia coli or bovine Ubiquitin), increases apoptosis in serum-deprived P19 mouse teratocarcinoma cells. The effect is dose-dependent, with a bell-shaped curve and peak activity at 10−9 M (maximal effect: 62.9 ± 17.7% increase, mean ± SD, n = 10) and is specifically inhibited by a polyclonal antibody raised against the synthetic protein. On the other hand, when the same cells are exponentially growing,M. tuberculosis Hsp10 increases cell proliferation 0 with a bell-shaped dose-response curve and a moderate decrease in potency (peak-activity at 10−8-10−7 M, with a 43.7 ± 8.1% increase, mean ± SD, n = 3). Therefore, it appears that this bacterial protein can exert two opposite effects, behaving either as a death-or as a growth-promoting factor, depending on the conditions of the target. Society for In Vitro Biology, 1996 mycobacteria nationallicence granuloma nationallicence early pregnancy factor nationallicence apoptosis nationallicence cell growth nationallicence Galli Grazia Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut Ghezzi Pietro Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut Mascagni Paolo Italfarmaco Research Center, Cinisello Balsamo, 20092, Milan, Italy aut Marcucci Fabrizio Italfarmaco Research Center, Cinisello Balsamo, 20092, Milan, Italy aut Fratelli Maddalena Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 446-450 1071-2690 32:7<446 1996 32 11626 https://doi.org/10.1007/BF02723008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Galli Grazia Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut NATIONALLICENCE 700 1- Ghezzi Pietro Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut NATIONALLICENCE 700 1- Mascagni Paolo Italfarmaco Research Center, Cinisello Balsamo, 20092, Milan, Italy aut NATIONALLICENCE 700 1- Marcucci Fabrizio Italfarmaco Research Center, Cinisello Balsamo, 20092, Milan, Italy aut NATIONALLICENCE 700 1- Fratelli Maddalena Istituto di Ricerche Farmacologiche "Mario Negri”, Via Eritrea 62, 20157, Milan, Italy aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 446-450 1071-2690 32:7<446 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer