caa a22 4500 477081363 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF02723006 doi (NATIONALLICENCE)springer-10.1007/BF02723006 Santarén Juan Centro de Biología Molecular "Severo Ochoa,” CSIC-UAM, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain aut Two-dimensional gel analysis of proteins in cell lines from the central nervous system of larval Drosophila [Elektronische Daten] [Juan Santarén] Summary: High resolution two-dimensional gel electrophoresis was used to quantitatively analyze the patterns of protein synthesis in three different clones of a nerve cell line (ML-DmBG2) ofDrosophila melanogaster. When patterns of pulse-labeled proteins of the three different clones were compared, I observed quantitative variations affecting the rate of synthesis by twofold or more in 25-30% of the polypeptides and qualitative differences, always affecting less than 2% of the polypeptides. Patterns of protein synthesis were analyzed during the 24 d of culture, revealing both quantitative (increase or decrease; 40%) and qualitative (presence or absence; 3%) differences. More than 70 proteins synthesized in these cultures were secreted into the medium. Among them were two major groups of acidic proteins which disappeared with culture time. When cell lines and intact central nervous systems were compared, large differences in protein synthesis were observed. In fact, only 20% of the synthesized proteins were common to both isolated cells grownin vitro and the original nervous systemin vivo. Society for In Vitro Biology, 1996 Drosophila nationallicence central nervous system nationallicence 2-D gels nationallicence cell lines nationallicence In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 434-440 1071-2690 32:7<434 1996 32 11626 https://doi.org/10.1007/BF02723006 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723006 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Santarén Juan Centro de Biología Molecular "Severo Ochoa,” CSIC-UAM, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/7(1996-07-01), 434-440 1071-2690 32:7<434 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer