caa a22 4500 477081401 CHVBK 20180405111450.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02723679 doi (NATIONALLICENCE)springer-10.1007/BF02723679 Establishment and characterization of a bovine mammary epithelial cell line with unique properties [Elektronische Daten] [Boris Zavizion, Marilyn van Duffelen, Warren Schaeffer, Ioannis Politis] Summary: Clonal cell lines (BME-UV) were established from primary epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T antigen. The BME-UV cells have undergone more than 300 population doublings and produce intranuclear large T antigen. At low confluency, growing islands of cells are apparent exhibiting the characteristic cobblestone morphology of epithelial cells. The BME-UV cells expressed functional markers such as microvilli and desmosomes and biochemical markers of mammary epithelial cells such as a repertoire of cytokeratins. The BME-UV cells are capable of synthesizing low levels of α-lactalbumin and α8l (50 ng/ml of medium/24 h). One of the cell lines, BME-UV1 showed enhanced proliferation in the presence of epidermal growth factor (EGF) and insulinlike growth factor I (IGF-I). The BME-UV1 cell line is the only known bovine mammary epithelial cell line responsive to EGF. The BME-UV cells grown on collagen at low confluency are capable of developing very long projections that most likely allow for communication between cells at a distance from each other. The BME-UV cells may become a valid model system to examine bovine mammary epithelial proliferation and differentiation and cell-to-cell communication. Society for In Vitro Biology, 1996 immortalization nationallicence T-antigen nationallicence Zavizion Boris Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut van Duffelen Marilyn Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut Schaeffer Warren Department of Microbiology and Molecular Genetics, University of Vermont, 05405, Burlington, Vermont aut Politis Ioannis Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 138-148 1071-2690 32:3<138 1996 32 11626 https://doi.org/10.1007/BF02723679 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723679 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zavizion Boris Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut NATIONALLICENCE 700 1- van Duffelen Marilyn Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut NATIONALLICENCE 700 1- Schaeffer Warren Department of Microbiology and Molecular Genetics, University of Vermont, 05405, Burlington, Vermont aut NATIONALLICENCE 700 1- Politis Ioannis Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 138-148 1071-2690 32:3<138 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 477081401 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer