caa a22 4500 477081452 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02723683 doi (NATIONALLICENCE)springer-10.1007/BF02723683 Modified antigen-binding of human antibodies with glycosylation variations of the light chains produced in sugar-limited human hybridoma cultures [Elektronische Daten] [Hirofumi Tachibana, Kim Jiyoun, Kiyotaka Taniguchi, Yoshitaka Ushio, Kiichiro Teruya, Kazuhiro Osada, Yuichi Inoue, Sanetaka Shirahata, Hiroki Murakami] Summary: We have characterized the effects of serum andN-acetylglucosamine in a glucose-deprived condition on the glycosylation of antibody light chains, as well as the resulting biological properties of those antibodies. We have chosen for our investigation the human hybridoma lines producing monoclonal antibodies reactive to lung adenocarcinoma. Each antibody possess aN-glycosylated carbohydrate chain in the hypervariable region of the light chains. When the cell lines were grown in the absence of glucose, variant light chains with varying molecular masses were found to be secreted. Analysis of these light chains produced in a glucose-deprived condition revealed that the changed molecular-mass of the variant light chains is due to different glycosylation. Addition ofN-acetylglucosamine or fetal calf serum to the glucose-free medium led to the creation of other light chains that exhibit increased antigen binding activity. Society for In Vitro Biology, 1996 human monoclonal antibody nationallicence light chain nationallicence glycosylation nationallicence antigen-binding nationallicence monosaccharide nationallicence Tachibana Hirofumi Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Jiyoun Kim Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Taniguchi Kiyotaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Ushio Yoshitaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Teruya Kiichiro Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Osada Kazuhiro Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Inoue Yuichi Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Shirahata Sanetaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut Murakami Hiroki Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 178-183 1071-2690 32:3<178 1996 32 11626 https://doi.org/10.1007/BF02723683 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723683 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tachibana Hirofumi Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Jiyoun Kim Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Taniguchi Kiyotaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ushio Yoshitaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Teruya Kiichiro Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Osada Kazuhiro Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Inoue Yuichi Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Shirahata Sanetaka Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Murakami Hiroki Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 178-183 1071-2690 32:3<178 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer