caa a22 4500 477081479 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF02723681 doi (NATIONALLICENCE)springer-10.1007/BF02723681 Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media [Elektronische Daten] [Hans Haugland, Ole-Bjørn Tysnes] Summary: Malignant features in three glioma cell lines were studied in four defined media of various complexity. The cell lines D37MG, D54MG, and GaMG were able to grow in monolayer culture in all media examined, and as multicellular tumor spheroids in the two most nutrient-rich media. In the defined media, none of the cell lines were able to migrate in a migration assay on poly-D-lysine-coated plastic surfaces. Flow cytometric analysis of the GaMG cell line demonstrated no medium-dependent selection of subclones of glioma cells in spheroids cultured for 30 d. Morphological diversity of spheroids varied according to the supplementation of the media. The capacity of glioma cells to invade cellular rat brain aggregates was intact in the media examined. However, glioma migration was severely inhibited by the lack of specific serum components. This study demonstrates that glioma growth and invasion was heterogenously preserved in the defined media used. Depending on the assay to be used in the study of glioma cell behavior, the degree of medium supplementation has to be considered. Society for In Vitro Biology, 1996 glioma nationallicence defined media nationallicence monolayer nationallicence spheroid nationallicence proliferation nationallicence migration nationallicence invasion nationallicence Haugland Hans The Gade Institute, Department of Pathology, University of Bergen, N-5021, Bergen, Norway aut Tysnes Ole-Bjørn Department of Neurology, University of Bergen, N-5021, Bergen, Norway aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 159-166 1071-2690 32:3<159 1996 32 11626 https://doi.org/10.1007/BF02723681 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723681 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haugland Hans The Gade Institute, Department of Pathology, University of Bergen, N-5021, Bergen, Norway aut NATIONALLICENCE 700 1- Tysnes Ole-Bjørn Department of Neurology, University of Bergen, N-5021, Bergen, Norway aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/3(1996-03-01), 159-166 1071-2690 32:3<159 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer