caa a22 4500 477081487 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02724048 doi (NATIONALLICENCE)springer-10.1007/BF02724048 Lectin-induced alterations on the proliferation of three human prostatic cancer cell lines [Elektronische Daten] [Isabelle Camby, Thierry Janssen, Robert De Decker, Michel Petein, Gil Raviv, Jean-Lambert Pasteels, Robert Kiss, André Danguy] Summary: While lectins are known to influence the cell growth of several types of normal and neoplastic tissues, their roles in the case of prostatic cancer cells remain relatively unexplored. In the present work, we report thein vitro influence of five lectins, namely peanut (PNA), wheat germ (WGA), Concanavalin A (Con A),Griffonia simplicifolia (GSA-IA4), andPhaseolus vulgaris (PHA-L) agglutinins, on the cell proliferation of one androgen-sensitive (LNCaP) and two androgen-insensitive (PC-3 and DU 145) human prostatic cancer cell lines cultured in either 10% or 1% fetal bovine serum (FBS)-supplemented media. The cell proliferation was assessed by means of the colorimetric 3-(4,5-dimethythiazol-2-yle)2,5-diphenyltetrazolium bromide. (MTT) assay. Four lectin concentrations were tested (i.e., 0.1, 1, 10, and 100 μg/ml) at five experimental states (i.e., 2, 3, 5, 7, and 9 d following the addition of each lectin to the culture media). Our results demonstrated that the five lectins under study had a globally significant dose-dependent toxic effect on prostatic cancer cell proliferation. Nevertheless, low doses of GSA-IA4 and PHA-L significantly (P<0.05 toP<0.001) increased the cell proliferation of confluent PC-3 cells. Increasing the FBS from 1% to 10% in the culture media significantly antagonized lectin-induced toxicity in the three prostatic cell lines. In conclusion, the present data strongly suggest that some lectins might influence the proliferation of prostatic carcinoma cells. In addition, because lectins are present in our diet, and are able to pass into the systemic circulation and thus reach the prostate, the present results suggest that some lectins might exert an influence on prostate cancer growth under clinical conditions. Society for In Vitro Biology, 1996 wheat germ agglutinin (WGA) nationallicence peanut agglutinin (PNA) nationallicence Griffonia simplicifolia I-A4 agglutinin (GSA-IA4) nationallicence Phaseolus vulgaris agglutinin (PHA-L) nationallicence Concanavalin A (Con A) nationallicence in vitro nationallicence Camby Isabelle Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut Janssen Thierry Service d'Urologie, Hôpital Erasme, Université Libre de Bruxelles, Bruxelles, Belgium aut De Decker Robert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut Petein Michel Département de Pathologie, Institut J. Bordet, Bruxelles, Belgium aut Raviv Gil Service d'Urologie, Hôpital Erasme, Université Libre de Bruxelles, Bruxelles, Belgium aut Pasteels Jean-Lambert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut Kiss Robert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut Danguy André Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 633-639 1071-2690 32:10<633 1996 32 11626 https://doi.org/10.1007/BF02724048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02724048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Camby Isabelle Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Janssen Thierry Service d'Urologie, Hôpital Erasme, Université Libre de Bruxelles, Bruxelles, Belgium aut NATIONALLICENCE 700 1- De Decker Robert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Petein Michel Département de Pathologie, Institut J. Bordet, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Raviv Gil Service d'Urologie, Hôpital Erasme, Université Libre de Bruxelles, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Pasteels Jean-Lambert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Kiss Robert Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut NATIONALLICENCE 700 1- Danguy André Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 route de Lennik, 1070, Bruxelles, Belgium aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 633-639 1071-2690 32:10<633 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer