caa a22 4500 477081495 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02724047 doi (NATIONALLICENCE)springer-10.1007/BF02724047 Dura mater secretes soluble heparin-binding factors required for cranial suture morphogenesis [Elektronische Daten] [Lynne Opperman, Ralph Passarelli, Amber Nolen, Thomas Gampper, Kant Lin, Roy Ogle] Summary: Cranial sutures play a critical role in calvarial morphogenesis, serving as bone growth centers during skull enlargement. Defective suture morphogenesis, resulting in premature osseous obliteration of sutures and their failure to function appropriately, causes severe craniofacial anomalies. Previously published data demonstrated osseous obliteration of coronal suturesin vitro in the absence of dura mater and the rescue of sutures from osseous obliteration in rudiments cocultured with dura mater on the opposite sides of 0.45-μm polycarbonate filters. With thisin vitro culture system, experiments were designed to examine the nature of the soluble signal secreted by dura mater, required for maintaining intact sutures. The signal remained active in conditioned medium produced from dura mater, which was capable of rescuing coronal sutures from osseous obliteration in calvaria cultured without dura mater. When conditioned medium was segregated into heparin-binding and non-heparin-binding fractions, the signal capable of maintaining intact coronal sutures cosegregated with the heparin-binding component and remained functional in the absence of the non-heparin-binding component of conditioned medium. Evidence indicates that soluble, heparin-binding factors secreted by the dura mater act as osteoinhibitory signals at the suture site. Society for In Vitro Biology, 1996 development nationallicence in vitro nationallicence calvaria nationallicence rat nationallicence growth factors nationallicence Opperman Lynne Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut Passarelli Ralph Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut Nolen Amber Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut Gampper Thomas Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut Lin Kant Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut Ogle Roy Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 627-632 1071-2690 32:10<627 1996 32 11626 https://doi.org/10.1007/BF02724047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02724047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Opperman Lynne Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 700 1- Passarelli Ralph Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 700 1- Nolen Amber Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 700 1- Gampper Thomas Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 700 1- Lin Kant Department of Plastic Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 700 1- Ogle Roy Department of Neurological Surgery, Health Sciences Center, University of Virginia, Box 568, 22908, Charlottesville, Virginia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 627-632 1071-2690 32:10<627 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer