caa a22 4500 477081509 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02724045 doi (NATIONALLICENCE)springer-10.1007/BF02724045 Human and animal mesenchymal progenitor cells from bone marrow: Identification of serum for optimal selection and proliferation [Elektronische Daten] [Donald Lennon, Stephen Haynesworth, Scott Bruder, Neelam Jaiswal, Arnold Caplan] Summary: An undifferentiated subset of cells within the stromal cell population of bone marrow in postnatal mammals retains the capacity to differentiate along osteogenic, adipogenic, fibroblastic, and chondrogenic lines. These cells, which are referred to as mesenchymal stem cells (MSCs), can be maintainedin vitro and expanded in number through a process of subculturing. MSCs are maintained in culture in medium supplemented with 10% fetal bovine serum (FBS). It is believed that certain, as yet unidentified, serum components play critical roles in the attachment and proliferation of MSCs. Commercially available FBS is poorly characterized and may vary in composition and quality from lot to lot. This study describes a method for the selection of lots of FBS that best support maintenance of the undifferentiated state, mitotic expansion of MSCsin vitro, and retention of multilineage developmental potential in response to appropriate cues. Society for In Vitro Biology, 1996 fetal bovine serum nationallicence proliferation nationallicence mesenchymal stem cell nationallicence marrow nationallicence osteochondral potential nationallicence Lennon Donald Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut Haynesworth Stephen Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut Bruder Scott Department of Orthopaedics, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut Jaiswal Neelam Osiris Terapeutics, Inc., Baltimore, Maryland aut Caplan Arnold Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 602-611 1071-2690 32:10<602 1996 32 11626 https://doi.org/10.1007/BF02724045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02724045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lennon Donald Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut NATIONALLICENCE 700 1- Haynesworth Stephen Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut NATIONALLICENCE 700 1- Bruder Scott Department of Orthopaedics, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut NATIONALLICENCE 700 1- Jaiswal Neelam Osiris Terapeutics, Inc., Baltimore, Maryland aut NATIONALLICENCE 700 1- Caplan Arnold Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, 44106-7080, Cleveland, Ohio aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 602-611 1071-2690 32:10<602 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer