caa a22 4500 477081576 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02724050 doi (NATIONALLICENCE)springer-10.1007/BF02724050 DNA fingerprinting of human cell lines using PCR amplification of fragment length polymorphisms [Elektronische Daten] [Rui Yan, Mark Ottenbreit, Bharati Hukku, Michael Mally, Sharong Chou, Joseph Kaplan] Summary: Methods for monitoring cell line identification and authentication include species-specific immunofluorescence, isoenzyme phenotyping, chromosome analysis, and DNA fingerprinting. Most previous studies of DNA fingerprinting of cell lines have used restriction fragment length polymorphism analysis. In this study, we examined the utility of an alternative and simpler method of cell line DNA fingerprinting—polymerase chain reaction (PCR) amplification of fragment length polymorphisms. Fourteen human cell lines previously found by other methods to be either related or disparate were subjected to DNA fingerprinting by PCR amplification of selected fragment length polymorphism loci. Cell identification patterns by this method were concordant with those obtained by isoenzyme phenotyping and restriction fragment length polymorphism-DNA fingerprinting, and were reproducible within and between assays on different DNA extracts of the same cell line. High precision was achieved with electrophoretic separation of amplified DNA products on high resolution agarose or polyacrylamide gels, and with fragment length polymorphism (FLP) loci-specific "allelic ladders” to identify individual FLP alleles. Determination of the composite fingerprint of a cell line at six appropriately chosen fragment length polymorphism loci should achieve a minimum discrimination power of 0.999. The ability of PCR-based fragment length polymorphism DNA fingerprinting to precisely and accurately identify the alleles of different human cell lines at multiple polymorphic fragment length polymorphism loci demonstrates the feasibility of developing a cell line DNA fingerprint reference database as a powerful additional tool for future cell line identification and authentication. Society for In Vitro Biology, 1996 DNA fingerprinting nationallicence cell lines nationallicence polymerase chain reaction nationallicence Yan Rui Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut Ottenbreit Mark Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut Hukku Bharati Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut Mally Michael Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut Chou Sharong Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut Kaplan Joseph Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 656-662 1071-2690 32:10<656 1996 32 11626 https://doi.org/10.1007/BF02724050 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02724050 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Rui Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Ottenbreit Mark Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Hukku Bharati Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Mally Michael Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Chou Sharong Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Kaplan Joseph Cell Culture Laboratory, Children's Hospital of Michigan, 3901 Beaubien Boulevard, 48201, Detroit, Michigan aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 656-662 1071-2690 32:10<656 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer