caa a22 4500 477081584 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02724046 doi (NATIONALLICENCE)springer-10.1007/BF02724046 An in vitro , serum-free organ culture technique for the study of development and growth of the dermal skeleton in fish [Elektronische Daten] [Joseph Koumans, Jean-yves Sire] Summary: To develop a serum-free, chemically definedin vitro organ culture system enabling the study of epithelial-mesenchymal interactions in development and growth of fish dermal skeleton, we investigatedin vitro continuation of scale regeneration in the cichlid fishHemichromis bimaculatus. The culture medium in our system is based on Leibovitz medium (L-15) supplemented with vitamin C, additional amino acids and HEPES. With this basis medium, we examined the effects of all trans-retinoic acid, dexamethasone, and prostaglandin-E2 (PG-E2), factors known to exert an effect on development and growth of teeth and bone in mammalian culture systems, on thein vitro regeneration of scales. These effects were compared with those obtained by supplementation of the basis medium with newborn and fetal calf serum. To evaluate our culture system, the medium that allowed to mimick in the best possible way thein vivo regeneration of scales (i.e., the basis medium plus dexamethasone and PG-E2) was also tested on thein vitro development of teeth in the same fish species. Our serum-free, chemically defined organ culture system enablesin vitro development and growth of both scales and teeth. With this model culture system, it is possible to evaluate thein vitro effects of hormones, growth factors, and other substances on growth and development of dermal skeleton in fish. Society for In Vitro Biology, 1996 fish nationallicence skeleton nationallicence development nationallicence organ culture nationallicence serum free nationallicence Koumans Joseph URA CNRS 1137, Laboratoire d'Anatomie comparée, Université Paris 7, Case 7077, Equipe "Formations Squelettiques”, 2, place Jussieu, 75251, Paris cedex 05, France aut Sire Jean-yves URA CNRS 1137, Laboratoire d'Anatomie comparée, Université Paris 7, Case 7077, Equipe "Formations Squelettiques”, 2, place Jussieu, 75251, Paris cedex 05, France aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 612-626 1071-2690 32:10<612 1996 32 11626 https://doi.org/10.1007/BF02724046 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02724046 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koumans Joseph URA CNRS 1137, Laboratoire d'Anatomie comparée, Université Paris 7, Case 7077, Equipe "Formations Squelettiques”, 2, place Jussieu, 75251, Paris cedex 05, France aut NATIONALLICENCE 700 1- Sire Jean-yves URA CNRS 1137, Laboratoire d'Anatomie comparée, Université Paris 7, Case 7077, Equipe "Formations Squelettiques”, 2, place Jussieu, 75251, Paris cedex 05, France aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/10(1996-11-01), 612-626 1071-2690 32:10<612 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer