caa a22 4500 477081592 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02722946 doi (NATIONALLICENCE)springer-10.1007/BF02722946 Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids [Elektronische Daten] [Madhusudan Peshwa, Florence Wu, Harvey Sharp, Frank Cerra, Wei-Shou Hu] Summary: Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids. Society for In Vitro Biology, 1996 hepatocyte nationallicence spheroid nationallicence multicellular aggregate nationallicence time lapse microscopy nationallicence Peshwa Madhusudan Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut Wu Florence Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut Sharp Harvey Department of Pediatrics, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut Cerra Frank Department of Surgery, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut Hu Wei-Shou Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 197-203 1071-2690 32:4<197 1996 32 11626 https://doi.org/10.1007/BF02722946 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722946 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peshwa Madhusudan Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut NATIONALLICENCE 700 1- Wu Florence Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut NATIONALLICENCE 700 1- Sharp Harvey Department of Pediatrics, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut NATIONALLICENCE 700 1- Cerra Frank Department of Surgery, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut NATIONALLICENCE 700 1- Hu Wei-Shou Department of Chemical Engineering and Materials Science, University of Minnesota, 55455-0132, Minneapolis, Minnesota aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 197-203 1071-2690 32:4<197 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer