caa a22 4500 477081649 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02722953 doi (NATIONALLICENCE)springer-10.1007/BF02722953 Comparative susceptibility of a canine cell line and bluetongue virus susceptible cell lines to a bluetongue virus isolate pathogenic for dogs [Elektronische Daten] [Marius Ianconescu, Geoffrey Akita, Bennie Osburn] Summary: Recently, bluetongue virus (BLU) serotype 11 was detected in diseased dogs that had been inoculated with live attenuated vaccine contaminated with this serotype of bluetongue virus (Akita et al., 1994). For various laboratory tests, BLU can be propagated in different cell cultures. No information was found in the literature about the possibility of propagating this virus in canine cells. To determine whether the BLU isolate from the contaminated canine vaccine (BLU-vac) is unique in its ability to replicate in canine cells, this virus was studied in parallel with U.S. prototype strains of BLU (serotypes 2, 10, 11, 13, and 17), in hamster lung (HmLu-1) and canine kidney (MDCK) cell cultures. In HmLu-1 cell cultures, the BLU-vac produced cytopathic effect (CPE) of the same type as the U.S. prototype BLU strains by 4 to 6 d postinoculation. In MDCK cell cultures, all of the BLU strains tested were able to replicate but did not produce CPE. The BLU-inoculated MDCK cells became persistently infected, and these cultures continued to produce infectious BLU even after six serial passages over 2 1/2 mo. In none of these cultures was CPE observed. In mixed cultures containing both HmLu-1 and MDCK cells, CPE first affected the HmLu-1 islands; subsequently, CPE spread also to the areas with MDCK cells. The silent persistent infection of the MDCK cells with BLU indicates that more stringent screening of the cells used in the production of live vaccines for various contaminating viruses is necessary. Society for In Vitro Biology, 1996 bluetongue virus nationallicence dog nationallicence cell culture nationallicence vaccine nationallicence Ianconescu Marius Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut Akita Geoffrey Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut Osburn Bennie Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 249-254 1071-2690 32:4<249 1996 32 11626 https://doi.org/10.1007/BF02722953 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722953 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ianconescu Marius Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut NATIONALLICENCE 700 1- Akita Geoffrey Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut NATIONALLICENCE 700 1- Osburn Bennie Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, 95616, Davis, California aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 249-254 1071-2690 32:4<249 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer