caa a22 4500 477081657 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02722949 doi (NATIONALLICENCE)springer-10.1007/BF02722949 Effect of anoxia and reoxygenation on antioxidant enzyme activities in immortalized brain endothelial cells [Elektronische Daten] [Olivier Rabin, Michèle Piciotti, Katy Drieu, Jean-Marie Bourre, Françoise Roux] Summary: The effects of anoxia and reoxygenation on major antioxidant enzyme activities were investigatedin vitro in immortalized rat brain endothelial cells (RBE4 cells). A sublethal anoxic period of 12 h was assessed for RBE4 cells using the neutral red uptake test. Anoxia markedly influenced the specific activity of catalase and superoxide dismutase, with no major effect on glutathione peroxidase or glutathione reductase. After 24 h postanoxia, the superoxide dismutase activity modulated by the presence or absence of oxygen returned to control value. Damage and recovery of RBE4 immortalized rat brain endothelial cells in culture after exposure to free radicals and other oxygen-derived species provides a usefulin vitro model to study anoxia-reoxygenation trauma at the cellular level. Society for In Vitro Biology, 1996 anoxia nationallicence immortalized endothelial cell nationallicence antioxidant enzymes nationallicence cell culture nationallicence rat brain endothelium nationallicence Rabin Olivier Laboratory of Neurosciences, Building 10-Room 6C103, National Institute of Aging, National Institute of Health, 20892, Bethesda, Maryland aut Piciotti Michèle INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut Drieu Katy Institut Henri Beaufour, 75016, Paris, France aut Bourre Jean-Marie INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut Roux Françoise INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 221-224 1071-2690 32:4<221 1996 32 11626 https://doi.org/10.1007/BF02722949 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722949 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rabin Olivier Laboratory of Neurosciences, Building 10-Room 6C103, National Institute of Aging, National Institute of Health, 20892, Bethesda, Maryland aut NATIONALLICENCE 700 1- Piciotti Michèle INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut NATIONALLICENCE 700 1- Drieu Katy Institut Henri Beaufour, 75016, Paris, France aut NATIONALLICENCE 700 1- Bourre Jean-Marie INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut NATIONALLICENCE 700 1- Roux Françoise INSERM U 26, Hôpital Fernand WIDAL, 75010, Paris, France aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 221-224 1071-2690 32:4<221 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer