caa a22 4500 47708169X CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02722950 doi (NATIONALLICENCE)springer-10.1007/BF02722950 Cytokine production by a megakaryocytic cell line [Elektronische Daten] [Balzer Sandrock, Karen Hudson, Douglas Williams, Michael Lieberman] Summary: The regulation of megakaryopoeisis by cytokines is not yet well understood. It is possible that autocrine loops are established during megakaryocyte growth and differentiation, aiding in the maturation of these cells. The CHRF-288-11 human megakaryoblastic cell line has been examined for cytokine production in growing cells and cells stimulated to differentiate by the addition of phorbol esters. It has been demonstrated that these cells produce RNA corresponding to the interleukins IL-1α, 1β, 3, 7, 8, and 11, granulocyte-macrophage colony stimulating factor (GM-CSF), stem cell factor (SCF), transforming growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α), interferon-α (INF-α), and basic fibroblast growth factor (bFGF). Additionaly, RNA corresponding to the receptors for IL-6, GM-CSF, SCF, INF-α,β, bFGF, and monocyte colony stimulating factor (M-CSF) were also expressed by the cells. The receptor for TNF-α was detected immunologically. Analysis at the protein level demonstrated that significant amounts of INF-α, TNF-α, GM-CSF, SCF, IL-1α, and a soluble form of the IL-6 receptor were produced by the cells. Addition of phorbol esters to CHRF-288-11 cells enhances their megakaryocytic phenotype; such treatment also results in increased secretion of INF-α, TNF-α, and GM-CSF. These results suggest that potential autocrine loops are established during the differentiation of CHRF-288-11 cells, which may alter the capability of the cell to differentiate. These findings are similar to those recently obtained for marrow-derived megakaryocytes (Jiang et al.) suggesting that CHRF-288-11 cells provide a useful model system for the study of cytokine release during megakaryocyte differentiation. Society for In Vitro Biology, 1996 cytokine nationallicence megakaryocyte nationallicence polymerase chain reaction nationallicence ELISA nationallicence Sandrock Balzer Department of Hematology-Oncology, Children's Hospital, 3333 Burnet Ave., 45229-3039, Cincinnati, Ohio aut Hudson Karen aut Williams Douglas Immunex Corporation, 51 University Street, 98101, Seattle, Washington aut Lieberman Michael Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Bethesda Avenue, 45267-0524, Cincinnati, Ohio aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 225-233 1071-2690 32:4<225 1996 32 11626 https://doi.org/10.1007/BF02722950 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722950 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sandrock Balzer Department of Hematology-Oncology, Children's Hospital, 3333 Burnet Ave., 45229-3039, Cincinnati, Ohio aut NATIONALLICENCE 700 1- Hudson Karen aut NATIONALLICENCE 700 1- Williams Douglas Immunex Corporation, 51 University Street, 98101, Seattle, Washington aut NATIONALLICENCE 700 1- Lieberman Michael Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Bethesda Avenue, 45267-0524, Cincinnati, Ohio aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 225-233 1071-2690 32:4<225 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer