caa a22 4500 477081703 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02722951 doi (NATIONALLICENCE)springer-10.1007/BF02722951 Drug-induced reversion of progression phenotype is accompanied by reversion of AP-1 phenotype in JB6 cells [Elektronische Daten] [V. Lavrovsky, Z. Dong, W. Ma, N. Colburn] Summary: Transformed JB6 cells can be stably reverted to nontransformed phenotype by AP-1 inhibiting glucocorticoid fluocinolone (FA) and cAMP elevator forskolin (FN), yielding stable revertants of promotion resistant (P−) and promotion sensitive (P+) phenotypes. AP-1 activity of nontransformed P− and P+ revertant clones was decreased under a variety of experimental conditions compared with their transformed counterparts. Moreover, AP-1 activity in P+ cells under anchorage-independent conditions was induced by 12-0-tetradecanoyl-phorbol-13-acetate (TPA) while AP-1 activity in the reverted P− cells was not induced, just as observed for the original P+ and P− variants. Taken together these data suggest that changes in AP-1 activity may be one key mediator not only of forward progression but also of reversion of tumor cells to nontransformed phenotype. In addition, the higher transfection efficiency of the new reverted P− and P+ cells renders them useful for studying the role of transcription factors in tumor promotion. Society for In Vitro Biology, 1996 transformed cells nationallicence revertant cells nationallicence AP-1 activity nationallicence Lavrovsky V. Laboratory of Viral Carcinogenesis, NCI-FCRDC, Building 560, Room 21-27, 21702-1201, Frederick, Maryland aut Dong Z. Biological Carcinogenesis and Development Program, PRI/DynCorp., NCI-FCRDC, 21702-1201, Frederick, Maryland aut Ma W. The Hormel Institute, University of Minnesota, 55912, Austin, Minnesota aut Colburn N. Laboratory of Viral Carcinogenesis, NCI-FCRDC, Building 560, Room 21-27, 21702-1201, Frederick, Maryland aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 234-237 1071-2690 32:4<234 1996 32 11626 https://doi.org/10.1007/BF02722951 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02722951 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lavrovsky V. Laboratory of Viral Carcinogenesis, NCI-FCRDC, Building 560, Room 21-27, 21702-1201, Frederick, Maryland aut NATIONALLICENCE 700 1- Dong Z. Biological Carcinogenesis and Development Program, PRI/DynCorp., NCI-FCRDC, 21702-1201, Frederick, Maryland aut NATIONALLICENCE 700 1- Ma W. The Hormel Institute, University of Minnesota, 55912, Austin, Minnesota aut NATIONALLICENCE 700 1- Colburn N. Laboratory of Viral Carcinogenesis, NCI-FCRDC, Building 560, Room 21-27, 21702-1201, Frederick, Maryland aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/4(1996-04-01), 234-237 1071-2690 32:4<234 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer