caa a22 4500 477081711 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02723051 doi (NATIONALLICENCE)springer-10.1007/BF02723051 Cardiogel: A biosynthetic extracellular matrix for cardiomyocyte culture [Elektronische Daten] [W. Vanwinkle, Mark Snuggs, L. Buja] Summary: Tissue-cultured neonatal cardiomyocytes can be successfully maintained in culture on a variety of extracellular matrix components such as laminin, fibronectin, and interstitial collagens (Types I and III).In vivo, however, cardiomyocytes (as well as many other cells) exist in a highly complex extracellular matrix environment composed of, in addition to the above three components, other proteins, proteoglycans, and growth factors. We have developed a procedure for culturing cardiomyocytes on a naturally occurring complete extracellular matrix, Cardiogel. This substrate, synthesized by cardiac fibroblasts, contains laminin, fibronectin, Types I and III collagen, and proteoglycans. When compared to cardiomyocytes grown on laminin alone or fibronectin alone, Cardiogel-supported cardiomyocytes adhere more rapidly after plating, exhibit spontaneous contractility earlier, undergo cytoskeletal and myofibrillar differentiation earlier, and grow larger than their counterparts. We suggest that their superior growth characteristics reflect the synergistic effect of numerous extracellular matrix components' signals in Cardiogel transduced by the cardiomyocyte cytoskeletal elements. Society for In Vitro Biology, 1996 myocardial tissue culture nationallicence cytoskeleton nationallicence confocal scanning laser immunofluorescence microscopy nationallicence Vanwinkle W. Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut Snuggs Mark Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut Buja L. Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 478-485 1071-2690 32:8<478 1996 32 11626 https://doi.org/10.1007/BF02723051 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723051 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vanwinkle W. Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Snuggs Mark Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut NATIONALLICENCE 700 1- Buja L. Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, 77030, Houston, Texas aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 478-485 1071-2690 32:8<478 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer