caa a22 4500 477081738 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02723053 doi (NATIONALLICENCE)springer-10.1007/BF02723053 Analysis of WT1 gene expression during mouse nephrogenesis in organ culture [Elektronische Daten] [Herman Yeger, Diane Forget, Jennifer Alami, Bryan Williams] Summary: The temporal and spatial expression patterns of the Wilms tumor gene, WT1, were studied during the organogenesis of the mouse kidneyin vitro. In situ hybridization and immunocytochemistry localized cellular expression of WT1 in whole kidney organ cultures to the induced metanephric mesenchyme and developing podocytes. Organ cultures were further characterized immunocytochemically with antibodies that specifically labeled the different tubular epithelial components and supporting mesenchyme of the developing nephrons. In organ cultures, the WT1 expression pattern could be visualized in induced metanephric mesenchyme and entire cell cohorts of differentiating podocytes. Expression of WT1 and cell specific markers were retained in short-term monolayer cultures of dissociated kidneys. The development of the metanephric kidneyin vitro involves a highly restricted temporal and spatial cellular expression pattern of WT1 which closely follows that observed in tissue sections from gestational kidney isolated during organogenesis in the mouse. Society for In Vitro Biology, 1996 nephrogenesis nationallicence WT1 nationallicence mouse organ culture nationallicence in situ hybridization nationallicence Yeger Herman Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut Forget Diane Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut Alami Jennifer Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut Williams Bryan Department of Cancer Biology, Cleveland Clinic Foundation Research Institute, 9500 Euclid Ave, 44195, Cleveland, Ohio aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 496-504 1071-2690 32:8<496 1996 32 11626 https://doi.org/10.1007/BF02723053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeger Herman Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Forget Diane Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Alami Jennifer Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Williams Bryan Department of Cancer Biology, Cleveland Clinic Foundation Research Institute, 9500 Euclid Ave, 44195, Cleveland, Ohio aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 496-504 1071-2690 32:8<496 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer