caa a22 4500 477081789 CHVBK 20180405111451.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02723054 doi (NATIONALLICENCE)springer-10.1007/BF02723054 Enhanced apoptosis in transformed human lung fibroblasts after exposure to sodium butyrate [Elektronische Daten] [Graham Thomas, Anna Henley, Tami Rowland, Animesh Sahai, Martin Griffin, Paul Birckbichler] Summary: Simian virus-transformed human cells, WI-38 VA13A, showed a dose-dependent induction of apoptosis and reduction in cell numbers after exposure to sodium butyrate. Apoptosis was confirmed by ApopTag staining, isolation of apoptotic envelopes, and immunofluorescent staining with an antibody specific for apoptotic envelopes. Examination of the cell cultures by phase contrast and fluorescent microscopy revealed the presence of enlarged cells that displayed a more flattened morphology and morphological changes in the nucleus of cells exposed to sodium butyrate. Cell proliferation assays showed control and sodium butyrate cultures were synthesizing DNA and excluded any cytotoxic effects of sodium butyrate. Flow cytometry results indicated an increase in the number of aneuploid cells following sodium butyrate treatment. There was a decrease in the percentage of cells in G2/M in the diploid populations, but an increase in the percentage of cells in G2/M in aneuploid populations. This humanin vitro model system suggests a mode of action for the therapeutic effects of sodium butyrate, which have been observed in the topical treatment of neoplastic cells and reversal of symptoms in ulcerative colitis: namely, the induction of apoptosis. Society for In Vitro Biology, 1996 transglutaminase nationallicence differentiation nationallicence cell cycle nationallicence human cells nationallicence Thomas Graham Department of Life Sciences, The Nottingham Trent University, NG11 8NS, Nottingham, England, UK aut Henley Anna Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut Rowland Tami Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut Sahai Animesh Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut Griffin Martin Department of Life Sciences, The Nottingham Trent University, NG11 8NS, Nottingham, England, UK aut Birckbichler Paul Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 505-513 1071-2690 32:8<505 1996 32 11626 https://doi.org/10.1007/BF02723054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomas Graham Department of Life Sciences, The Nottingham Trent University, NG11 8NS, Nottingham, England, UK aut NATIONALLICENCE 700 1- Henley Anna Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut NATIONALLICENCE 700 1- Rowland Tami Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut NATIONALLICENCE 700 1- Sahai Animesh Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut NATIONALLICENCE 700 1- Griffin Martin Department of Life Sciences, The Nottingham Trent University, NG11 8NS, Nottingham, England, UK aut NATIONALLICENCE 700 1- Birckbichler Paul Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104, Oklahoma aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/8(1996-09-01), 505-513 1071-2690 32:8<505 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer