caa a22 4500 477081843 CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF02723064 doi (NATIONALLICENCE)springer-10.1007/BF02723064 Cell cycle parameters in Aedes albopictus mosquito cells [Elektronische Daten] [Anna Gerenday, Ann Fallon] Summary: We have analyzed cell cycle parameters for theAedes albopictus C7-10 mosquito cell line, which has been systematically developed for somatic cell genetics, expression of transfected genes, and synthesis of hormone-inducible proteins. In rapidly cycling cells, we measured a generation time of 10-12 h. The duration of mitosis (M) was ≤1 h, and the DNA synthesis phase (S) required 6 h. UnlikeDrosophila melanogaster Kc cells, in which the G2 gap is substantially longer than G1, in C7-10 cells G1 and G2 each lasted approximately 2h. In these cells, the duration of both S and G2 was independent of the population doubling time, and the increase in population doubling time as cells approached confluency was due to prolongation of G1. When treated with the insect steroid hormone, 20-hydroxyecdysone, C7-10 mosquito cells complete the cycle in progress before undergoing a reversible arrest. Society for In Vitro Biology, 1996 Aedes albopictus nationallicence Drosophila melanogaster nationallicence insect nationallicence 20-hydroxyecdysone nationallicence mitosis nationallicence DNA synthesis nationallicence Gerenday Anna Department of Entomology, University of Minnesota, 1980 Folwell Avenue, 55108, St. Paul, Minnesota aut Fallon Ann Department of Entomology, University of Minnesota, 1980 Folwell Avenue, 55108, St. Paul, Minnesota aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 307-312 1071-2690 32:5<307 1996 32 11626 https://doi.org/10.1007/BF02723064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gerenday Anna Department of Entomology, University of Minnesota, 1980 Folwell Avenue, 55108, St. Paul, Minnesota aut NATIONALLICENCE 700 1- Fallon Ann Department of Entomology, University of Minnesota, 1980 Folwell Avenue, 55108, St. Paul, Minnesota aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 307-312 1071-2690 32:5<307 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer