caa a22 4500 477081851 CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF02723059 doi (NATIONALLICENCE)springer-10.1007/BF02723059 Development of a new method for obtaining osteoclasts from endosteal surfaces [Elektronische Daten] [Lisa May, Carol Gay] Summary: Techniques for the isolation of ahhighly pure population of viable osteoclasts are limited. For this reason, we developed an isolation procedure that results in a high yield of osteoclast-like cells, up to 92% pure, from 3-wk-old chicken tibias. The unique feature of the method is the migration of cells from marrow-free endosteal surfaces to vitronectin-coated plates. The cells retain the osteoclast phenotype and remain viable in culture for a minimum of 1 wk. The cells were characterized and compared to two populations of authentic avian osteoclasts, which were isolated on the basis of association with fibronectin-coated plates. The cells contained substantial amounts of tartrate-resistant acid phosphatase. Alkaline phosphatase levels were negligible, suggesting little contamination by osteoblasts. Response to parathyroid hormone, dibutyryl cyclic adenosine monophosphate, calcitonin, acetazolamide, 17β-estradiol, and prostaglandin E2 was evident, as detected by measuring acid production. The vitronectin-associating cells contained numerous mitochondria, had the ability to resorb bone in anin vitro bone slice assay, and specifically bound biotinylated vitronectin. At 5 d of culture, the cells demonstrated marginal multinuclearity, having two to three nuclei. A large number (∼1×106 cells/tibia) of viable cells that exhibit characteristics of authentic osteoclasts can be obtained by the method described. Potentially, this method could be applied to other species. Society for In Vitro Biology, 1996 osteoclast isolation nationallicence migratory osteoclasts nationallicence vitronectin-coated surfaces nationallicence osteoclast-like cell characterization nationallicence May Lisa Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania aut Gay Carol Department of Poultry Science, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 269-278 1071-2690 32:5<269 1996 32 11626 https://doi.org/10.1007/BF02723059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- May Lisa Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Gay Carol Department of Poultry Science, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 269-278 1071-2690 32:5<269 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer