caa a22 4500 47708186X CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF02723060 doi (NATIONALLICENCE)springer-10.1007/BF02723060 Human papilloma virus E6/E7 genes can expand the lifespan of human corneal fibroblasts [Elektronische Daten] [Donna Peters, Nathan Dowd, Curtis Brandt, Teresa Compton] Summary: Human corneal fibroblasts were infected with a retroviral delivery vector containing the E6 and E7 genes from human Papilloma virus type 16 in order to produce cell lines that have an expanded lifespan in culture. Morphologically, some of the trasfected corneal fibroblast lines appeared to have the normal spindle-shape morphology of diploid fibroblasts, whereas other lines appeared to have a more elongated morphology. All the cell lines were anchorage-dependent. Cells that had a normal morphology grew at a rate similar to normal diploid human corneal fibroblasts and had a population doubling time of 48 h. All E6/E7 expressing cell lines, regardless of morphology, produce types I, III, and V collagen, at levels similar to those observed in the parent corneal diploid fibroblast. These corneal fibroblast lines will be a usefulin vitro system to study collagen expression and fibril formation, as well as normal stroma development. These results also demonstrate that the use of E6/E7 genes to expand a cell's lifespan can be a powerful tool because it does not appear to alter either the growth rate of the cell or collagen expression. Society for In Vitro Biology, 1996 fibroblasts nationallicence collagen nationallicence human papilloma virus nationallicence cell culture nationallicence corneal stroma nationallicence Peters Donna Department of Laboratory Medicine and Pathology, 505 SMI, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut Dowd Nathan Department of Laboratory Medicine and Pathology, 505 SMI, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut Brandt Curtis Department of Ophthalmology and Visual Sciences, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut Compton Teresa Department of Medical Microbiology and Immunology, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 279-284 1071-2690 32:5<279 1996 32 11626 https://doi.org/10.1007/BF02723060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peters Donna Department of Laboratory Medicine and Pathology, 505 SMI, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut NATIONALLICENCE 700 1- Dowd Nathan Department of Laboratory Medicine and Pathology, 505 SMI, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut NATIONALLICENCE 700 1- Brandt Curtis Department of Ophthalmology and Visual Sciences, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut NATIONALLICENCE 700 1- Compton Teresa Department of Medical Microbiology and Immunology, University of Wisconsin, 1300 University Avenue, 53706, Madison, Wisconsin aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/5(1996-05-01), 279-284 1071-2690 32:5<279 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer