caa a22 4500 477081975 CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF02723042 doi (NATIONALLICENCE)springer-10.1007/BF02723042 Growth and propagation of normal rat intestinal epithelial cells [Elektronische Daten] [R. Odedra, C. Hart, J. Saunders, B. Getty, S. van de Wall, S. Sørensen, H. Embaye, R. Batt] Summary: A combination of mild proteolytic digestion and selective growth stimulation has been used to isolate and propagate adult rat intestinal epithelial cells with a finite life span. Growth of these cells on a variety of matrices and on mesenchymal cells has resulted in the expression of brush border enzymes including sucrase-isomaltase, aminopeptidase N, and alkaline phosphatase. Examination of the cells at the electron microscopic level has revealed that although these cells express key brush border enzymes, they do not have a fully formed brush border. These findings suggest that the expression of brush border enzymes and structural proteins represent distinct stages of enterocyte differentiation that are under separate transcriptional and temporal control. Society for In Vitro Biology, 1996 differentiation nationallicence intestinal epithelium nationallicence extracellular matrix nationallicence fibroblast growth factor nationallicence heparin nationallicence Odedra R. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut Hart C. Department of Medical Microbiology, University of Liverpool, UK aut Saunders J. Department of Genetics and Microbiology, University of Liverpool, UK aut Getty B. Department of Medical Microbiology, University of Liverpool, UK aut van de Wall S. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut Sørensen S. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut Embaye H. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut Batt R. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/2(1996-02-01), 107-115 1071-2690 32:2<107 1996 32 11626 https://doi.org/10.1007/BF02723042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Odedra R. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut NATIONALLICENCE 700 1- Hart C. Department of Medical Microbiology, University of Liverpool, UK aut NATIONALLICENCE 700 1- Saunders J. Department of Genetics and Microbiology, University of Liverpool, UK aut NATIONALLICENCE 700 1- Getty B. Department of Medical Microbiology, University of Liverpool, UK aut NATIONALLICENCE 700 1- van de Wall S. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut NATIONALLICENCE 700 1- Sørensen S. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut NATIONALLICENCE 700 1- Embaye H. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut NATIONALLICENCE 700 1- Batt R. Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, London aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/2(1996-02-01), 107-115 1071-2690 32:2<107 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer