caa a22 4500 477082017 CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF02723043 doi (NATIONALLICENCE)springer-10.1007/BF02723043 Expression of ICAM-1 on intact cartilage and isolated chondrocytes [Elektronische Daten] [J. Bujia, U. Behrends, N. Rotter, P. Pitzke, E. Wilmes, C. Hammer] Summary: A major factor in cellular cytotoxicity is the interaction between LFA-1 on leukocytes and ICAM-1 on targets. Because several inflammatory cartilage diseases are characterized by the presence of leukocyte infiltrates, the expression of ICAM-1 on human cartilage, cultured chondrocytes, and transplanted cartilage was investigated using monoclonal antibodies. Frozen tissue sections, chondrocytes in suspension, as well as total cellular mRNA were prepared from human cartilage samples. ICAM-1 expression was studied with two different monoclonal antibodies directed against ICAM-1 by immunohistochemical APAAP-staining and additional flow cytometric analyses. The expression of ICAM-1-mRNA in cartilage tissue was analyzed using the northern blot hybridization technique. Furthermore, chondrocytes were treated in culture with interleukin-1 (IL-1) and gamma-interferon (gamma-IFN). ICAM-1 expression after culture was quantified using flow cytometric analysis. We could detect ICAM-1 mRNA in cartilage tissue, however, the immunostaining of tissue sections using monoclonal antibodies did not give clear positive reactions. Isolated chondrocytes showed strongly positive staining patterns in comparison with adequate negative controls as assessed by flow cytometry. A dose-dependent increase of the expression of ICAM-1 on chondrocytes was observed when stimulated with IL-1 and gamma-IFN. Finally, two of the three studied transplanted autologous cartilage samples with advanced resorption showed the presence of ICAM-1 molecules as assessed by immunohistochemistry. This expression of ICAM-1 suggests that the molecule plays a role in severe cartilage inflammatory processes, where tissue damage leads to the exposure of chondrocyte surfaces. Society for In Vitro Biology, 1996 chondrocytes nationallicence ICAM-1 nationallicence IL-1 nationallicence gamma-IFN nationallicence immunohistochemistry nationallicence flow cytometry nationallicence northern blot analysis nationallicence Bujia J. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut Behrends U. GSF-Hämatologikum, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut Rotter N. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut Pitzke P. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut Wilmes E. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut Hammer C. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/2(1996-02-01), 116-122 1071-2690 32:2<116 1996 32 11626 https://doi.org/10.1007/BF02723043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02723043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujia J. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Behrends U. GSF-Hämatologikum, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Rotter N. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Pitzke P. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Wilmes E. Department of Otorhinolaryngology, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 700 1- Hammer C. Institute for Surgical Research, Ludwig-Maximilians-University of Munich, Klinikum Großhadern, Marchioninistraße 15, 81366, Munich, Germany aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 32/2(1996-02-01), 116-122 1071-2690 32:2<116 1996 32 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer