caa a22 4500 477082122 CHVBK 20180405111452.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF01922419 doi (NATIONALLICENCE)springer-10.1007/BF01922419 Tumor necrosis factor-α inhibits collagen synthesis in human and rat granulation tissue fibroblasts [Elektronische Daten] [K. Rapala, M. Vähä-Kreula, J. Heino, E. Vuorio, M. Laato] The purpose of the study was to examine the effects of tumor necrosis factor-α (TNF-α) on collagen gene expression in rat and human granulation tissue fibroblast cultures. The cells were exposed to 0.1, 1, 10, or 100 ng/ml of TNF-α, and the rate of collagen synthesis was measured as synthesis of protein-bound3H-hydroxyproline. Total cellular RNA was isolated from fibroblasts, and measurements of specific cellular RNAs from fibroblasts were performed by Northern blot hybridizations using32P-labeled cDNA probes. In cultures of rat granulation tissue fibroblasts TNF-α decreased3H-hydroxyproline production to about 75% of that in controls and it also decreased proα1(I) and proα1(III) collagen mRNA levels, maximally to 33% and 23% of the control levels, respectively. In cultures of human granulation tissue fibroblasts a similar inhibiting effect in the production of collagen was seen. TNF-α decreased the production of3H-hydroxyproline to 56% of the control value with a dose of 100 ng/ml also having an inhibiting effect on proα1(I) collagen mRNA levels of up to 43% of the control level. However, no effect was seen on proα1(III) collagen mRNA levels. Birkhäuser Verlag Basel, 1996 Tumor necrosis factor-α nationallicence wound healing nationallicence granulation tissue nationallicence collagne nationallicence collagen synthesis nationallicence fibroblast nationallicence Rapala K. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut Vähä-Kreula M. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut Heino J. Department of Medical Biochemistry, University of Turku, FIN-20520, Turku, (Finland) aut Vuorio E. Department of Medical Biochemistry, University of Turku, FIN-20520, Turku, (Finland) aut Laato M. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut Experientia Birkhäuser-Verlag 52/1(1996-01-01), 70-74 0014-4754 52:1<70 1996 52 18 https://doi.org/10.1007/BF01922419 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01922419 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rapala K. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut NATIONALLICENCE 700 1- Vähä-Kreula M. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut NATIONALLICENCE 700 1- Heino J. Department of Medical Biochemistry, University of Turku, FIN-20520, Turku, (Finland) aut NATIONALLICENCE 700 1- Vuorio E. Department of Medical Biochemistry, University of Turku, FIN-20520, Turku, (Finland) aut NATIONALLICENCE 700 1- Laato M. Department of Surgery, University of Turku, FIN-20520, Turku, (Finland) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/1(1996-01-01), 70-74 0014-4754 52:1<70 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer