caa a22 4500 47708222X CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969723 doi (NATIONALLICENCE)springer-10.1007/BF01969723 In situ hybridization analysis of globin mRNAs in the primitive erythroid cells of the chick embryo [Elektronische Daten] [L. Fucci, U. Galderisi, M. Piscopo, R. del Gaudio, G. Geraci] The possibility that the minor embryonic chick hemoglobins might be present in a particular subgroup of primitive erythroid cells has been investigated by in situ hybridization. Probe to detect the mRNA for the αA globin chain of the minor embryonic hemoglobin was used, and the results of the hybridization were compared with those obtained using as probes the cDNAs for total globin mRNAs. All erythroid cells circulating in a 4-day-old chick embryo gave positive signals with both probes at an approximately constant ratio. This shows that all cells contain a similar assortment of hemoglobin types, excluding the possibility that a subgroup might contain the minor primitive hemoglobins exclusively. However, the cells are not homogeneous, since about 10% of them show a distinctly higher concentration of mRNA of all globin types. Birkhäuser Verlag, 1996 αA nationallicence mRNAs nationallicence primitive erythroid cells nationallicence chick embryo nationallicence in situ hybridization nationallicence Fucci L. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut Galderisi U. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut Piscopo M. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut del Gaudio R. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut Geraci G. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 535-539 0014-4754 52:6<535 1996 52 18 https://doi.org/10.1007/BF01969723 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969723 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fucci L. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut NATIONALLICENCE 700 1- Galderisi U. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut NATIONALLICENCE 700 1- Piscopo M. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut NATIONALLICENCE 700 1- del Gaudio R. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut NATIONALLICENCE 700 1- Geraci G. Department of Genetics, General and Molecular Biology, University of Naples, Via Mezzocannone 8, I-80134, Naples, (Italy) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 535-539 0014-4754 52:6<535 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer