caa a22 4500 477082254 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969736 doi (NATIONALLICENCE)springer-10.1007/BF01969736 Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system [Elektronische Daten] [H. Baker, B. DeAngelis, O. Frank, M. Khalil, S. Hutner, E. Baker] Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems. Birkhäuser Verlag, 1996 Glutathione nationallicence free radicals nationallicence antioxidants nationallicence oxidant stressors nationallicence buthionine nationallicence menadione nationallicence MD : menadione sodium bisulphite nationallicence BSO : buthionine sulphoximine nationallicence GSH : gluthathione nationallicence GSSG : gluthathione disulphide nationallicence NAC : N-acetyl-L-cysteine nationallicence OTC : 2-oxo-4-thiazolidine carboxylic acid nationallicence DTT : dithiothreitol nationallicence Baker H. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut DeAngelis B. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut Frank O. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut Khalil M. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut Hutner S. Haskins Laboratories at Pace University, 10038, New York, New York, USA aut Baker E. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 597-599 0014-4754 52:6<597 1996 52 18 https://doi.org/10.1007/BF01969736 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969736 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baker H. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut NATIONALLICENCE 700 1- DeAngelis B. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut NATIONALLICENCE 700 1- Frank O. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut NATIONALLICENCE 700 1- Khalil M. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut NATIONALLICENCE 700 1- Hutner S. Haskins Laboratories at Pace University, 10038, New York, New York, USA aut NATIONALLICENCE 700 1- Baker E. Departments of Preventive Medicine and Community Health and Medicine, New Jersey Medical School, 65 Bergen Street, 07107, Newark, New Jersey, USA aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 597-599 0014-4754 52:6<597 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer