caa a22 4500 477082270 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969727 doi (NATIONALLICENCE)springer-10.1007/BF01969727 Differences in membrane ion transport between hepatocytes from the periportal and the pericentral areas of the liver lobule [Elektronische Daten] [A. Sillau, N. Escobales, C. Juarbe] We studied the Na+/K+ pump, Na+/K+ ATPase activity, and oxygen consumption (QO2) in hepatocytes isolated from the periportal (PH) and pericentral (CH) regions of the liver lobule, to provide an insight into the functional properties of these cells. Na+/K+ pump activity was determined using86Rb+ (a functional analog of K+) and ouabain, a specific inhibitor of this transport system. Our results indicate the the Na+/K+, pump and Na+/K+ ATPase activity are significantly lower in CH than in PH, although basal ouabain-sensitive (OS) QO2 was negligible in both of these cell preparations. However, OSQO2 was significantly lower in CH than in PH when the Na+/K+ pump was activated using the ionophore nystatin in a Na+-containing medium. These results indicate that the differences in membrane ion transport exist between hepatocytes from different locations of the liver lobule. Birkhäuser Verlag, 1996 Ion transport nationallicence hepatocytes nationallicence liver nationallicence Sillau A. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut Escobales N. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut Juarbe C. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 554-557 0014-4754 52:6<554 1996 52 18 https://doi.org/10.1007/BF01969727 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969727 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sillau A. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut NATIONALLICENCE 700 1- Escobales N. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut NATIONALLICENCE 700 1- Juarbe C. Department of Physiology, School of Medicine, University of Puerto Rico, GPO Box 5067, 00936, San Juan, Puerto Rico, USA aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 554-557 0014-4754 52:6<554 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer