caa a22 4500 477082351 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969726 doi (NATIONALLICENCE)springer-10.1007/BF01969726 Immunocytochemical reaction of a haemocyanin antibody in the midgut gland of Nautilus (Cephalopoda, Tetrabranchiata) [Elektronische Daten] [P. Ruth, W. Blum, J. Bille] The branchial gland of the dibranchiate cephalopods is described as the site of haemocyanin synthesis. Because there is no equivalent to this organ in tetrabranchiate cephalopods the localization of haemocyanin synthesis remained unknown for a long time. In this study we could confirm the conclusions from prelimnary investigations concerning the copper content of the midgut gland ofNautilus, which gave the first indications for a possible localization of haemocyanin synthesis in this organ. We developed a polyclonal antibody againstNautilus haemocyanin, tested its specificity, and used it on ultra-thin sections of the tissue of the midgut gland. It could be shown that there is a clear imunogold precipitation only on the triangular basal cells in the terminal alveoli. All the other types of cell in this organ were free of any immunoreactivity. It can be supposed that the triangular basal cells in the terminal alveoli of the midgut gland are the sites of haemocyanin synthesis inNautilus. Birkhäuser Verlag, 1996 Haemocyanin synthesis nationallicence Cephalopoda nationallicence Nautilus nationallicence midgut gland nationallicence immunocytochemistry nationallicence Ruth P. Institut für Allgemeine und Spezielle Zoologie, Stephanstraße 24, D-35390, Gießen, (Germany) aut Blum W. Institut für Allgemeine Botanik, Senckenbergstraße 17, D-35390, Gießen, (Germany) aut Bille J. Institut für Allgemeine Botanik, Senckenbergstraße 17, D-35390, Gießen, (Germany) aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 549-553 0014-4754 52:6<549 1996 52 18 https://doi.org/10.1007/BF01969726 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969726 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruth P. Institut für Allgemeine und Spezielle Zoologie, Stephanstraße 24, D-35390, Gießen, (Germany) aut NATIONALLICENCE 700 1- Blum W. Institut für Allgemeine Botanik, Senckenbergstraße 17, D-35390, Gießen, (Germany) aut NATIONALLICENCE 700 1- Bille J. Institut für Allgemeine Botanik, Senckenbergstraße 17, D-35390, Gießen, (Germany) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 549-553 0014-4754 52:6<549 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer