caa a22 4500 477082467 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969725 doi (NATIONALLICENCE)springer-10.1007/BF01969725 Purification and some properties of hemagglutinin from the Myxomycete, Physarum polycephalum [Elektronische Daten] [M. Yokota, K. Nitta] A new hemagglutinin was isolated from the plasmodium ofPhysarum polycephalum by salting out with ammonium sulphate followed by chromatography on DE-32, DEAE-Toyopearl and hydroxyapatite. This hemagglutinin, named physarumin, was purified 1000-fold over crude extracts. The molecular weight of physarumin was determined to be 22,000 by size exclusion chromatography on Bio-Gel P-60 and 8,700 by SDS-polyacrylamide gel electrophoresis. Physarumin agglutinated rabbit, guinea pig, horse and human erythrocytes. Physarumin-induced hemagglutination was inhibited by fetuin and α1 glycoprotein, but not by commercially available mono-and disaccharides. Hemagglutinating activity was blocked by EDTA, and was restored by adding Ca2+ but not by Mg2+. Birkhäuser Verlag, 1996 Myxomycete nationallicence Physarum nationallicence hemagglutinin nationallicence Ca2+-dependent lectin nationallicence Yokota M. Department of Health Sciences, Asahikawa College of Hokkaido University of Education, Hokumon-cho 9, 070, Asahikawa, (Japan) aut Nitta K. Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Komatsushima 4-4-1, Aoba-ku, 981, Sendai, (Japan) aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 544-548 0014-4754 52:6<544 1996 52 18 https://doi.org/10.1007/BF01969725 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969725 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yokota M. Department of Health Sciences, Asahikawa College of Hokkaido University of Education, Hokumon-cho 9, 070, Asahikawa, (Japan) aut NATIONALLICENCE 700 1- Nitta K. Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Komatsushima 4-4-1, Aoba-ku, 981, Sendai, (Japan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 544-548 0014-4754 52:6<544 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer