caa a22 4500 477082491 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01969737 doi (NATIONALLICENCE)springer-10.1007/BF01969737 Antibacterial effect of 2-hydroxy- N -(3,4-dimethyl-5-isoxazolyl)-1,4-naphthoquinone-4-imine on Staphylococcus aureus [Elektronische Daten] [P. Bogdanov, I. Albesa, N. Sperandeo, C. Luna, M. de Bertorello] The mechanism by which a new naphthoquinone derivative, the 2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1,4-naphthoquinone-4-imine (INQI-E) has antibacterial effect againstStaphylococcus aureus was studied. The interaction of INQI-E with the bacteria was followed by absorption spectroscopy at 323 and 490 nm. The absorption band of INQI-E at 490 nm undergoes a hypochromic shift with a decrease of intensity. This effect was found to be reversible by oxygenation during the first hours of incubation. The participation of an oxidation-reduction process related to the respiratory chain was demonstrated by oxygen consumption. An increase in O2 uptake and inhibition ofS. aureus growth was observed. Experiments with three inhibitors of the respiratory chain demonstrated that the pathway induced by INQI-E was antimycin-resistant and KCN- and salicylhydroxamic acid (SHAM)-sensitive, which suggests that INQI-E is capable of diverting the normal electron flow to an alternate superoxide-producing route. On the other hand, experiments with Tiron, a specific scavenger of superoxide, hindered the effect of INQI-E againstS. aureus, indicating that the inhibitory growth effect of this quinone-imine is mainly due to the production of the cytotoxic superoxide radical. Birkhäuser Verlag, 1996 Staphylococcus aureus nationallicence isoxazolylnaphthoquinone nationallicence antibacterial effect nationallicence superoxide-producing pathway nationallicence Bogdanov P. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut Albesa I. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut Sperandeo N. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut Luna C. Fisiología Vegetal, Facultad de Ciencias Exactas, Universidad Nacional de Córdoba, (Argentina) aut de Bertorello M. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut Experientia Birkhäuser-Verlag 52/6(1996-06-01), 600-604 0014-4754 52:6<600 1996 52 18 https://doi.org/10.1007/BF01969737 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01969737 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bogdanov P. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut NATIONALLICENCE 700 1- Albesa I. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut NATIONALLICENCE 700 1- Sperandeo N. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut NATIONALLICENCE 700 1- Luna C. Fisiología Vegetal, Facultad de Ciencias Exactas, Universidad Nacional de Córdoba, (Argentina) aut NATIONALLICENCE 700 1- de Bertorello M. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Suc. 16 C.C. 61, 5016, Córdoba, (Argentina) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/6(1996-06-01), 600-604 0014-4754 52:6<600 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer