caa a22 4500 477082513 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01938876 doi (NATIONALLICENCE)springer-10.1007/BF01938876 Age-related changes in gene expression in the rat brain revealed by differential display [Elektronische Daten] [M. Salehi, M. Hodgkins, B. Merry, M. Goyns] We have used the polymerase chain reaction (PCR)-based technique of differential display to analyse changes in gene expression during ageing of the rat brain. In this approach we have compared three young adult (6 months) with three old adult (20 months) animals. RNA preparations from the homogenised brains were subjected to reverse transcriptase (RT)-PCR using 36 different combinations of primer pairs. Any PCR product which was consistently found to be more prominent in the three young brains compared to the three old brains, and vice versa, was scored as potentially representing a gene which was differentially expressed during the ageing of this tissue. Out of a possible 2000+PCR products we identified 44 that might represent genes that exhibit differential expression during ageing of the rat brain. An initial screen of these fragments, by Southern-blotting the PCR products and hybridising them with cDNA probes derived from either young or old brain RNA preparations, indicated that 40% of them represented genes that were differentially expressed. This approach is likely to prove invaluable for identifying cohorts of genes that show differential expression during the ageing process. Birkhäuser Verlag, 1996 Ageing nationallicence rat nationallicence brain nationallicence gene expression nationallicence differential display nationallicence Salehi M. Institute for Cancer Studies, Sheffield University Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut Hodgkins M. Institute for Cancer Studies, Sheffield University Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut Merry B. Institute of Human Ageing/Environmental and Evolutionary Biology, Liverpool University, P.O. Box 147, L69 3BX, Liverpool, UK aut Goyns M. School of Health Sciences, University of Sunderland, Fleming Building, Wharncliffe Street, SR2 3SD, Sunderland, UK aut Experientia Birkhäuser-Verlag 52/9(1996-09-01), 888-891 0014-4754 52:9<888 1996 52 18 https://doi.org/10.1007/BF01938876 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01938876 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salehi M. Institute for Cancer Studies, Sheffield University Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 700 1- Hodgkins M. Institute for Cancer Studies, Sheffield University Medical School, Beech Hill Road, S10 2RX, Sheffield, UK aut NATIONALLICENCE 700 1- Merry B. Institute of Human Ageing/Environmental and Evolutionary Biology, Liverpool University, P.O. Box 147, L69 3BX, Liverpool, UK aut NATIONALLICENCE 700 1- Goyns M. School of Health Sciences, University of Sunderland, Fleming Building, Wharncliffe Street, SR2 3SD, Sunderland, UK aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/9(1996-09-01), 888-891 0014-4754 52:9<888 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer