caa a22 4500 477082521 CHVBK 20180405111453.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01938868 doi (NATIONALLICENCE)springer-10.1007/BF01938868 Freeze-induced shrinkage of individual cells and cell-to-cell propagation of intracellular ice in cell chains from salivary glands [Elektronische Daten] [W. Berger, B. Uhrík] The formation of intracellular ice (IIF), usually a lethal event to be avoided when cryopreserving cells, should, however, be enforced during the cryosurgical destruction of tumour cells. IIF has been investigated so far only in single cells in suspension. Because cells in tissues cannot be successfully cryopreserved, in contrast to single cells in suspension, the mechanism of IIF in tissues may depend on factors that facilitate IIF. We studied IIF in cell strands from salivary glands, which represent a simple form of a tissue. Their cells are connected by channels responsible for intercellular communication. A substantial fraction of cell dehydration during freezing occurs before cells are encapsulated by ice, and the degree of this pre-ice-front shrinkage appears to influence IIF. In strands with coupled cells IIF spread from one cell to adjacent cells in a sequential manner with short delays (200-300 ms), suggesting cell-to-cell propagation via intercellular channels. In strands pretreated with decoupling agents (dinitrophenol, heptanol), sequential IIF was absent. Instead, formation of ice was random, with longer and variable delays between consecutive darkenings indicating IIF. Results suggest that the mechanism of IIF spread, and consequently the degree of cryodamage in tissue, can be influenced by the presence of intercellular channels (gap junctions). Birkhäuser Verlag, 1996 Salivary glands nationallicence cell strands nationallicence pre-ice-front shirnkage nationallicence intercellular communication nationallicence freeze-thaw cycle nationallicence intracellular ice nationallicence Berger W. I. Physiologisches Institut, Universität des Saarlandes, LKH, Bau 59, D-66424, Homburg, (Germany) aut Uhrík B. Institute of Molecular Physiology and Genetics, Slovak Acaemy of Sciences, Vlárska 5, 833 34, Bratislava, (Slovakia) aut Experientia Birkhäuser-Verlag 52/9(1996-09-01), 843-850 0014-4754 52:9<843 1996 52 18 https://doi.org/10.1007/BF01938868 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01938868 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berger W. I. Physiologisches Institut, Universität des Saarlandes, LKH, Bau 59, D-66424, Homburg, (Germany) aut NATIONALLICENCE 700 1- Uhrík B. Institute of Molecular Physiology and Genetics, Slovak Acaemy of Sciences, Vlárska 5, 833 34, Bratislava, (Slovakia) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/9(1996-09-01), 843-850 0014-4754 52:9<843 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer