caa a22 4500 477082564 CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01938879 doi (NATIONALLICENCE)springer-10.1007/BF01938879 Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide of Porphyromonas gingivalis [Elektronische Daten] [H. Shimauchi, T. Ogawa, H. Uchida, J. Yoshida, H. Ogoh, T. Nozaki, H. Okada] Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced byP. gingivalis LPS was incompletely inhibited by polymyxin B.P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared withEscherichia coli LPS. Furthermore,P. gingivalis LPS, but notE. coli LPS, induced definite IL-6 production in C3H/HeJ mice.P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinatedP. gingivalis LPS, similarly toE. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. ThusP. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells. Birkhäuser Verlag, 1996 Porphyromonas gingivalis nationallicence lipopolysaccharide (LPS) nationallicence C3H/HeJ mice nationallicence B-cells nationallicence protein phosphorylation nationallicence cytokine production nationallicence Shimauchi H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut Ogawa T. Department of Oral Microbiology, Faculty of Dentistry, Osaka University, 565, Suita, Osaka, (Japan) aut Uchida H. Department of Oral Microbiology, Faculty of Dentistry, Osaka University, 565, Suita, Osaka, (Japan) aut Yoshida J. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut Ogoh H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut Nozaki T. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut Okada H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut Experientia Birkhäuser-Verlag 52/9(1996-09-01), 909-917 0014-4754 52:9<909 1996 52 18 https://doi.org/10.1007/BF01938879 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01938879 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimauchi H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Ogawa T. Department of Oral Microbiology, Faculty of Dentistry, Osaka University, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Uchida H. Department of Oral Microbiology, Faculty of Dentistry, Osaka University, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Yoshida J. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Ogoh H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Nozaki T. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 700 1- Okada H. Department of Periodontology and Endodontology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, 565, Suita, Osaka, (Japan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/9(1996-09-01), 909-917 0014-4754 52:9<909 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer