caa a22 4500 477082629 CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01938875 doi (NATIONALLICENCE)springer-10.1007/BF01938875 Bombyxin gene expression in tissues other than brain detected by reverse transcriptionpolymerase chain reaction (RT-PCR) and in situ hybridization [Elektronische Daten] [M. Iwami, A. Tanaka, N. Hano, S. Sakurai] Bombyxin is a 5 kDa insulin-related peptide produced in four pairs of medial neurosecretory cells in the brain of the silkmothBombyx mori. We demonstrate here the presence of bombyxin mRNA in tissues other than brain: ganglia, epidermis, testis, ovary, fat body, silk gland, Malpighian tubule, midgut, and hindgut of theBombyx fifth instar larvae. Bombyxin mRNA was detected by Oligotex reverse transcription-polymerase chain reaction (RT-PCR), a rapid and simple procedure of reverse transcription-PCR, and in situ hybridization. The Oligotex RT-PCR method effectively eliminated the contaminating DNA in RNA samples and amplified bombyxin mRNA efficiently. In situ hybridization of theBombyx ovary clearly demonstrated the localization of the bombyxin mRNA in the ovariole. The present study is the first demonstration of expression of brain neurosecretory peptide in tissues other than the central nervous system in insects at RNA level. Birkhäuser Verlag, 1996 Insulin nationallicence insulin-like growth factor nationallicence Bombyx mori nationallicence ovary nationallicence neurosecretory peptide nationallicence Oligotex reverse transcription-polymerase chain reaction nationallicence Iwami M. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut Tanaka A. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut Hano N. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut Sakurai S. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut Experientia Birkhäuser-Verlag 52/9(1996-09-01), 882-887 0014-4754 52:9<882 1996 52 18 https://doi.org/10.1007/BF01938875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01938875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwami M. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut NATIONALLICENCE 700 1- Tanaka A. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut NATIONALLICENCE 700 1- Hano N. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut NATIONALLICENCE 700 1- Sakurai S. Department of Biology, Faculty of Science, Kanazawa University, 920-11, Kanazawa, (Japan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/9(1996-09-01), 882-887 0014-4754 52:9<882 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer