caa a22 4500 477082645 CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01938871 doi (NATIONALLICENCE)springer-10.1007/BF01938871 Effect of a dipeptide inhibiting ubiquitin-mediated protein degradation on nerve-dependent limb regeneration in the newt [Elektronische Daten] [C. Taban, H. Hondermarck, R. Bradshaw, B. Boilly] The dipeptide Leu-Ala, which inhibits ubiquitin-mediated protein degradation, has been shown to act in vitro as an inhibitor of neurite outgrowth of PC12 cells (Hondermarck et al. [1992] Biochem. Biophys. Res. Commun.189: 280). Using agarose beads as vehicles, we tested, in vivo, the effect of this dipeptide (and the inactive inverse, Ala-Leu, as a control) on limb regeneration in the newt (Triturus cristatus), a nerve-dependent developmental process. Leu-Ala inhibited the growth of mid-bud blastemas without altering blastema differentiation, while Ala-Leu had no effect. Cytological observations of dipeptide-treated blastemas using Bodian staining or neurofilament antibodies showed that all the blastema tissues were unmodified except with regard to innervation. Leu-Ala-treated blastemas were devoid of nerve fibers in the epidermal cap, while the mesenchyme distal to the dipeptide impregnated bead exhibited fewer nerve fibers than did Ala-Leu-treated blastemas, which were similar to the control nontreated blastemas. Thus, Leu-Ala, in reducing blastema innervation, inhibits its growth in the same manner as surgical denervation. Birkhäuser Verlag, 1996 Newt nationallicence limb regeneration nationallicence nerve regeneration nationallicence ubiquitin-mediated proteolysis nationallicence ubiquitin ligase (E3) nationallicence Taban C. aut Hondermarck H. Centre de Biologie Cellulaire, Unité ‘Dynamique des Cellules Embryonnaires et Cancéreuses', SN3, Université de Lille 1, F-59655, Villeneuve d'Ascq Cedex, (France) aut Bradshaw R. Department of Biological Chemistry, College of Medicine, University of California, 92717, Irvine, California, USA aut Boilly B. Centre de Biologie Cellulaire, Unité ‘Dynamique des Cellules Embryonnaires et Cancéreuses', SN3, Université de Lille 1, F-59655, Villeneuve d'Ascq Cedex, (France) aut Experientia Birkhäuser-Verlag 52/9(1996-09-01), 865-870 0014-4754 52:9<865 1996 52 18 https://doi.org/10.1007/BF01938871 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01938871 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taban C. aut NATIONALLICENCE 700 1- Hondermarck H. Centre de Biologie Cellulaire, Unité ‘Dynamique des Cellules Embryonnaires et Cancéreuses', SN3, Université de Lille 1, F-59655, Villeneuve d'Ascq Cedex, (France) aut NATIONALLICENCE 700 1- Bradshaw R. Department of Biological Chemistry, College of Medicine, University of California, 92717, Irvine, California, USA aut NATIONALLICENCE 700 1- Boilly B. Centre de Biologie Cellulaire, Unité ‘Dynamique des Cellules Embryonnaires et Cancéreuses', SN3, Université de Lille 1, F-59655, Villeneuve d'Ascq Cedex, (France) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/9(1996-09-01), 865-870 0014-4754 52:9<865 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer