caa a22 4500 47708267X CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01925566 doi (NATIONALLICENCE)springer-10.1007/BF01925566 Apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pig ovaries [Elektronische Daten] [N. Manabe, Y. Imai, H. Ohno, Y. Takahagi, M. Sugimoto, H. Miyamoto] The porcine antral follicles, 3-6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation, estimated by electrophoresis, were noted in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation (r=0.987) between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs. Birkhäuser Verlag, 1996 Apoptosis nationallicence Ca2+/Mg2+-dependent endonuclease nationallicence cumulus cell nationallicence granulosa cell nationallicence pig nationallicence Manabe N. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Imai Y. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Ohno H. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Takahagi Y. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Sugimoto M. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Miyamoto H. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut Experientia Birkhäuser-Verlag 52/7(1996-07-01), 647-651 0014-4754 52:7<647 1996 52 18 https://doi.org/10.1007/BF01925566 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01925566 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manabe N. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 700 1- Imai Y. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 700 1- Ohno H. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 700 1- Takahagi Y. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 700 1- Sugimoto M. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 700 1- Miyamoto H. Department of Animal Science, Kyoto University, 606-01, Kyoto, (Japan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/7(1996-07-01), 647-651 0014-4754 52:7<647 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer