caa a22 4500 47708270X CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01925578 doi (NATIONALLICENCE)springer-10.1007/BF01925578 Insect hormones in vertebrates: Anabolic effects of 20-hydroxyecdysone in Japanese quail [Elektronische Daten] [K. Sláma, K. Koudela, J. Tenora, A. Maťhová] Ecdysteroids are hormones controlling cell proliferation, growth and the developmental cycles of insects and other invertebrates1. They are occasionally present in various unrelated plants for no apparent reason; no phytohormonal function has yet been identified. In certain cases, ecdysteroids are accumulated to high levels in leaves, roots or seeds. Some ecdysteroid-containing plants have been known as medicinal plants for centuries. One of them,Leuzea carthamoides Iljin (Asteraceae), growing in Central Asia, contains 0.4% ecdysteroid in dry roots and 2% in seeds. A pharmacological preparation from this plant, ‘Ecdisten', is already available as a commercial preparation for its anabolic, tonic and other effects, for medical use (review2). It remained problematic, however, whether ecdysteroids were truly responsible for these effects, becauseLeuzea contains a number of other biologically active compounds in addition to ecdysteroids. We extracted and purified ecdysteroids from the seeds ofLeuzea. With 6 g of 96% 20-hydroxyecdysone (20E), we made a large-scale feeding assay with Japanese quail to find out whether ecdysteroid alone could duplicate the anabolic effects of the seeds. We found that the 96% ecdysteroid increased the mass of the developing quails in a dose-dependent manner, with the rate of increase proportional to the ecdysteroid content in the seeds; there was a 115% increase in living mass with 100 mg kg−1 of pure 20E compared with 109.5% increase with 100-180 mg kg−1 20E equivalents in the seeds. We conclude that the plethora of growth-promoting, vitamin-like effects induced in vertebrates byLeuzea is mediated by ecdysteroids. Birkhäuser Verlag, 1996 Ecdysteroids nationallicence ecdysone nationallicence 20-hydroxyecdysone nationallicence polyhydroxylated sterols nationallicence anabolic effects nationallicence Coturnix japonica (Aves) nationallicence Leuzea carthamoides (Asteraceae) nationallicence Sláma K. Institute of Entomology, Czech Academy of Sciences, Drnovská 507, 16100, Praha 6, (Czech Republic) aut Koudela K. Department of Animal Physiology, Agricultural University, Prague, (Czech Republic) aut Tenora J. Faculty of Medicine, Palack University, Olomouc, (Czech Republic) aut Maťhová A. Institute of Entomology, Czech Academy of Sciences, Branišovská 31, 37005 Č, Budějovice, (Czech Republic) aut Experientia Birkhäuser-Verlag 52/7(1996-07-01), 702-706 0014-4754 52:7<702 1996 52 18 https://doi.org/10.1007/BF01925578 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01925578 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sláma K. Institute of Entomology, Czech Academy of Sciences, Drnovská 507, 16100, Praha 6, (Czech Republic) aut NATIONALLICENCE 700 1- Koudela K. Department of Animal Physiology, Agricultural University, Prague, (Czech Republic) aut NATIONALLICENCE 700 1- Tenora J. Faculty of Medicine, Palack University, Olomouc, (Czech Republic) aut NATIONALLICENCE 700 1- Maťhová A. Institute of Entomology, Czech Academy of Sciences, Branišovská 31, 37005 Č, Budějovice, (Czech Republic) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/7(1996-07-01), 702-706 0014-4754 52:7<702 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer