caa a22 4500 477082823 CHVBK 20180405111454.0 cr unu---uuuuu 170330e19960701xx s 000 0 eng 10.1007/BF01925568 doi (NATIONALLICENCE)springer-10.1007/BF01925568 Lee J. Department of Physiology and Biophysics, H.S.C., SUNY, 11794-8661, Stony Brook, New York, USA aut The Na/K pump, resting potential and selective permeability in canine Purkinje fibres at physiologic and room temperatures [Elektronische Daten] [J. Lee] All mammalian cells maintain a resting potential generated by ions moving down concentration gradients. In excitable cells, the inside potential is negative relative to outside. In order to maintain this electrochemical gradient, the sodium potassium (Na/K) pump actively transports out three sodium ions for every two potassium ions it brings in. This process generates a net outward current and thus hyperpolaizes the resting potential. I employed dihydroouabain (DHO) to inhibit the Na/K pump and thus measure its contribution to the resting potential. It contributed 9.0 mV at 34°C and 3.8 mV at 25°C. The PK/PNa ratios were calculated at both temperatures before and after subtracting the Na/K pump contribution. These ratios also suggested a decreased contribution of the Na/K pump under hypothermia. Taken together, these results suggest that the pump contribution to the resting potential is more significant at physiologic temperatures (34°C) than at room temperature (25°C), and that estimates of selective permeability can only be accurately obtained after assessing and eliminating the Na/K pump contribution to the resting potential. Birkhäuser Verlag, 1996 Na/K pump nationallicence resting potential nationallicence selective permeability nationallicence Purkinje fibres nationallicence dihydroouabain nationallicence Experientia Birkhäuser-Verlag 52/7(1996-07-01), 657-660 0014-4754 52:7<657 1996 52 18 https://doi.org/10.1007/BF01925568 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01925568 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Lee J. Department of Physiology and Biophysics, H.S.C., SUNY, 11794-8661, Stony Brook, New York, USA aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/7(1996-07-01), 657-660 0014-4754 52:7<657 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer