caa a22 4500 477082890 CHVBK 20180405111454.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF01952108 doi (NATIONALLICENCE)springer-10.1007/BF01952108 Vacuolar H+-ATPase: From mammals to yeast and back [Elektronische Daten] [N. Nelson, D. Klionsky] Vacuolar H+-adenosine triphosphatase (V-ATPase) is composed of distinct catalytic (V1) and membrane (V0) sectors containing several subunits. The biochemistry of the enzyme was mainly studied in organelles from mammalian cells such as chromaffin granules and clathrin-coated vesicles. Subsequently, mammalian cDNAs and yeast genes encoding subunits of V-ATPase were cloned and sequenced. The sequence information revealed the relation between V- and F-ATPases that evolved from a common ancestor. The isolation of yeast genes encoding subunits of V-ATPase opened an avenue for molecular biology studies of the enzyme. Because V-ATPase is present in every known eukaryotic cell and provides energy for vital transport systems, it was anticipated that disruption of genes encoding V-ATPase subunits would be lethal. Fortunately, yeast cells can survive the absence of V-ATPase by ‘drinking' the acidic medium. So far only yeast cells have been shown to be viable without an active V-ATPase. In contrast to yeast, mammalian cells may have more than one gene encoding each of the subunits of the enzyme. Some of these genes encode tissue- and/or organelle-specific subunits. Expression of these specific cDNAs in yeast cells may reveal their unique functions in mammalian cells. Following the route from mammals to yeast and back may prove useful in the study of many other complicated processes. Birkhäuser Verlag, 1996 Vacuolar H+-ATPase nationallicence biogenesis nationallicence assembly nationallicence proton pump nationallicence bovine nationallicence yeast nationallicence Nelson N. Department of Biochemistry, Tel Aviv University, Ramat Aviv, 69978, Tel Aviv, Israel aut Klionsky D. Section of Microbiology, University of California, 95616, Davis, California, USA aut Experientia Birkhäuser-Verlag 52/12(1996-12-01), 1101-1110 0014-4754 52:12<1101 1996 52 18 https://doi.org/10.1007/BF01952108 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01952108 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nelson N. Department of Biochemistry, Tel Aviv University, Ramat Aviv, 69978, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Klionsky D. Section of Microbiology, University of California, 95616, Davis, California, USA aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/12(1996-12-01), 1101-1110 0014-4754 52:12<1101 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer