caa a22 4500 477082912 CHVBK 20180405111455.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF01952102 doi (NATIONALLICENCE)springer-10.1007/BF01952102 Baker A. Centre for Plant Biochemistry and Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut In vitro systems in the study of peroxisomal protein import [Elektronische Daten] [A. Baker] Our level of understanding of peroxisome biogenesis in comparison with other cellular organelles is rudimentary, yet the fragments of information available indicate that the targeting and import of peroxisomal proteins occur by fundamentally different mechanisms. Genetic studies have identified a number of genes required for peroxisome assembly, but in most cases the functions of the gene products remain unknown. In vitro protein translocation systems have played a prominent role in unravelling the biochemistry of protein translocation into other organelles. This review considers some of the requirements for establishing a bona fide peroxisomal import assay and discusses the findings which have emerged as a result of using such experimental systems. Birkhäuser Verlag, 1996 Peroxisomes nationallicence glyoxysomes nationallicence in vitro protein translocation assay nationallicence targeting signals nationallicence organelle biogenesis nationallicence Experientia Birkhäuser-Verlag 52/12(1996-12-01), 1055-1062 0014-4754 52:12<1055 1996 52 18 https://doi.org/10.1007/BF01952102 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01952102 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Baker A. Centre for Plant Biochemistry and Biotechnology, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/12(1996-12-01), 1055-1062 0014-4754 52:12<1055 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer