caa a22 4500 477083161 CHVBK 20180405111455.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01920713 doi (NATIONALLICENCE)springer-10.1007/BF01920713 Oxidative modifications of low-density lipoproteins (LDL) by the human endothelial cell line EA.hy 926 [Elektronische Daten] [M. Pech-Amsellem, I. Myara, I. Pico, C. Mazière, J. Mazière, N. Moatti] Modifications of LDL by the EA.hy 926 cell line were compared to those generated by human umbilical vein endothelial cells (HUVEC). Thiobarbituric acid reactive substances (TBARS) index values (TBARS sample/TBARS cell-free control ratio) were 2.64±0.18 (m±SE, n=11) and 3.12±0.24 (n=11), for HUVEC and EA.hy 926, respectively. The percentage of the most electronegative modified LDL fraction (fraction C), assessed by using an ion-exchange chromatographic method based on fast protein liquid chromatography (FPLC), represented 14±3% (n=34) and 22±13% (n=10) of total modified LDL in HUVEC and EA.hy 926, respectively. LDL modified by both cell lines showed increased agarose electrophoretic mobility and apo B100 fragmentation on SDS-PAGE. None of the results were significantly different between the two cell lines. Superoxide anion production was 0.12±0.04 (n=11) and 0.07±0.01 nmol/min/mg cell protein (n=11) in HUVEC and EA.hy 926, respectively. Cell-specific effects on LDL were abrogated in cysteine-free medium. Moreover, cell-modified LDL were similarly degraded by J774 macrophage-like cells. We conclude that EA.hy 926 cells are a good model for investigating endothelial cell-induced modifications of LDL. Advantages include ready availability and less individual variability than with HUVEC. Birkhäuser Verlag, 1996 LDL peroxidation nationallicence low-density lipoprotein nationallicence endothelial cell nationallicence EA.hy 926 nationallicence thiol nationallicence atherosclerosis nationallicence FPLC nationallicence LDL : low-density lipoprotein nationallicence HAT : 100 μM hypoxanthine/0.4 μM aminopterin/16 μM thymidine nationallicence FCS : fetal calf serum nationallicence TBARS : thiobarbituric acid-reactive substances (lipid peroxidation products) nationallicence MDA : malondialdehyde nationallicence FPLC : fast protein liquid chromatography nationallicence cpm : counts per minute nationallicence Pech-Amsellem M. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut Myara I. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut Pico I. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut Mazière C. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut Mazière J. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut Moatti N. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut Experientia Birkhäuser-Verlag 52/3(1996-03-01), 234-238 0014-4754 52:3<234 1996 52 18 https://doi.org/10.1007/BF01920713 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01920713 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pech-Amsellem M. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut NATIONALLICENCE 700 1- Myara I. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut NATIONALLICENCE 700 1- Pico I. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut NATIONALLICENCE 700 1- Mazière C. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut NATIONALLICENCE 700 1- Mazière J. Faculté de Médecine Saint-Antoine, Laboratoire de Biochimie Appliquée, 27, rue Chaligny, F-75012, Paris, France aut NATIONALLICENCE 700 1- Moatti N. Faculté de Pharmacie, Laboratoire de Biochimie Appliquée, F-92296, Châtenay-Malabry aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/3(1996-03-01), 234-238 0014-4754 52:3<234 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer