caa a22 4500 477083242 CHVBK 20180405111455.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF01920709 doi (NATIONALLICENCE)springer-10.1007/BF01920709 17β-estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes [Elektronische Daten] [G. Nikezić, A. Horvat, N. Nedeljković, J. Martinović] Effects of 17β-estradiol (E2) in vitro on Na-dependent Ca2+ efflux from, and depolarization-induced Ca2+ uptake into, the nerve cell were studied with the use of synaptosomes isolated from the brain stem, mesencephalic reticular formation (MRF), caudate nucleus and the hippocampus of long-term ovariectomized adult female rats. It was found that E2 (1) at a concentration of 10 nM or lower, stimulates Na-dependent Ca2+ efflux in the caudate nucleus and hippocampus, and does not affect the efflux in MRF and brain stem; (2) at concentrations above 10 nM has no effect on the Ca2+ efflux in any of the four structures investigated; and (3) produces a biphasic effect on the depolarization-induced Ca2+ uptake, increasing it in all structures except MRF at 10 nM concentration, and decreasing it at concentrations higher than 10 nM, irrespective of the structure investigated. These results suggest that E2, acting at extranuclear sites, modulates synaptic transmission via alterations of Ca2+ transport mechanisms in nerve endings. Birkhäuser Verlag, 1996 Estradiol nationallicence calcium transport nationallicence synaptosomes nationallicence brain nationallicence rat nationallicence Nikezić G. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut Horvat A. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut Nedeljković N. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut Martinović J. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut Experientia Birkhäuser-Verlag 52/3(1996-03-01), 217-220 0014-4754 52:3<217 1996 52 18 https://doi.org/10.1007/BF01920709 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01920709 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nikezić G. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut NATIONALLICENCE 700 1- Horvat A. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut NATIONALLICENCE 700 1- Nedeljković N. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut NATIONALLICENCE 700 1- Martinović J. Laboratory for Molecular Biology and Endocrinology, Vinča Institute for Nuclear Sciences, P.O. Box 522, YU-11001, Belgrade, Yugoslavia aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/3(1996-03-01), 217-220 0014-4754 52:3<217 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer