caa a22 4500 477083544 CHVBK 20180405111456.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919520 doi (NATIONALLICENCE)springer-10.1007/BF01919520 Direct sequencing versus cloned amplicon sequencing in HIV-1 diagnosis [Elektronische Daten] [H. Petry, K. Pekrun, K. Wäse, I. Schedel, W. Lüke, G. Hunsmann] Conclusions: HIV-1-specific sequences could easily be amplified from the DNA of peripheral blood lymphocytes or from the viral RNA present in the plasma. A detailed characterization of the resulting amplicons requires sequencing. Here we have shown that the sequencing strategy depends on the variability of the region of interest. The higher the variability of a distinct region the more a cloning step is necessary prior to sequencing to obtain reliable information. Conversely, direct sequencing analysis is usually sufficient to record changes of conserved viral sequences, e.g. during therapy with reverse transcriptase inhibitors. Birkhäuser Verlag, 1996 Petry H. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut Pekrun K. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut Wäse K. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut Schedel I. Medizinische Hochschule Hannover, Konstanty-Gutschow-Str. 6, D-30625, Hannover, Germany aut Lüke W. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut Hunsmann G. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 303-304 0014-4754 52:4<303 1996 52 18 https://doi.org/10.1007/BF01919520 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919520 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petry H. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Pekrun K. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Wäse K. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Schedel I. Medizinische Hochschule Hannover, Konstanty-Gutschow-Str. 6, D-30625, Hannover, Germany aut NATIONALLICENCE 700 1- Lüke W. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut NATIONALLICENCE 700 1- Hunsmann G. Institute for Immunology and Virology, German Primate Center, Kellnerweg 4, D-37077, Göttingen, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 303-304 0014-4754 52:4<303 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer