caa a22 4500 477083595 CHVBK 20180405111456.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919517 doi (NATIONALLICENCE)springer-10.1007/BF01919517 Detection of varicella zoster virus DNA in human tissue by standard and nested polymerase chain reaction [Elektronische Daten] [C. Mainka, M. Wolff] Results and conclusion: The sensitivity of the PCR assay was determined with cloned VZV DNA. About 200 copies of the target sequence were necessary for detectable amplification by standard PCR and less than 20 copies by nested PCR. Out of 24 human trigeminal ganglia five tested positive for VZV DNA by standard PCR (21%), in eleven cases VZV DNA was detectable using nested PCR (46%). Sequences specific for VZV could be detected in PMBC from children with acute varicella up to six days after the onset of rash by standard (one child) or nested (three children) PCR. This confirms that at the time of haematogenous spread before and during the rash viral DNA can be found within the mononuclear cells. Thus the use of nested primers enhances the sensitivity of the assay and allows the detection of only a few genomic copies of viruses in human tissues. Birkhäuser Verlag, 1996 Mainka C. Institute of Microbiology and Virology, University of Witten/Herdecke, Stockumer Str. 10, D-58448, Witten, Germany aut Wolff M. Institute of Microbiology and Virology, University of Witten/Herdecke, Stockumer Str. 10, D-58448, Witten, Germany aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 300-301 0014-4754 52:4<300 1996 52 18 https://doi.org/10.1007/BF01919517 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919517 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mainka C. Institute of Microbiology and Virology, University of Witten/Herdecke, Stockumer Str. 10, D-58448, Witten, Germany aut NATIONALLICENCE 700 1- Wolff M. Institute of Microbiology and Virology, University of Witten/Herdecke, Stockumer Str. 10, D-58448, Witten, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 300-301 0014-4754 52:4<300 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer