caa a22 4500 477083730 CHVBK 20180405111456.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919526 doi (NATIONALLICENCE)springer-10.1007/BF01919526 PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1 [Elektronische Daten] [B. Schweiger, C. Kücherer, C. Fleischer, H. v. Spreckelsen, P. Zablocki-Kaiser, G. Pauli] Conclusions: The application of PCR and techniques confirming the specificity of PCR products in routine diagnostic laboratories, requires that the procedures are simple and reproducible. The microtitre plate assay we described for detection of HIV-1 is sensitive, simple, rapid and reproducible. The DEIA test is perfectly compatible with standard enzyme linked immunosorbent assay equipment, permitting the processing of a large number of samples. Moreover, the ability to analyze DNA extracted from dried blood specimens, as demonstrated in this study, allows the long-term storage of blood samples even at elevated temperatures and after transport over long distances. Birkhäuser Verlag, 1996 Schweiger B. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut Kücherer C. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut Fleischer C. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut v. Spreckelsen H. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut Zablocki-Kaiser P. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut Pauli G. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 308-309 0014-4754 52:4<308 1996 52 18 https://doi.org/10.1007/BF01919526 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919526 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schweiger B. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 700 1- Kücherer C. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 700 1- Fleischer C. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 700 1- v. Spreckelsen H. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 700 1- Zablocki-Kaiser P. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 700 1- Pauli G. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 308-309 0014-4754 52:4<308 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer