caa a22 4500 477083757 CHVBK 20180405111456.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919542 doi (NATIONALLICENCE)springer-10.1007/BF01919542 Güner E. Moda cad. 256/22, 81300 Kadiköy, Istanbul, (Turkey) aut Complement evasion by the Lyme disease spirochete Borrelia burgdorferi grown in host-derived tissue co-cultures: role of fibronectin in complement-resistance [Elektronische Daten] [E. Güner] The effectiveness of complement-mediated killing ofBorrelia burgdorferi, the causative agent of Lyme disease, in the presence of host-derived tissues was studied. Second and high passage forms ofB. burgdorferi 297 isolate were grown in a LEW/N rat joint tissue co-culture system and in artificial BSK medium. Guinea pig complement and third week immune serum from hamsters with experimental Lyme disease were added to the cultures. Both high and low passage borrelia grown in BSK medium died and did not revive after 3 weeks incubation in BSK medium. However, 5-12% of tissue co-cultured borrelia survived the first complement-mediated lysis. Repeated re-growth and lysis cycles in tissue co-culture resulted in isolation of an 85% complement-resistant population ofB. burgdorferi. Joint tissue culture supernatant collected on the third day of tissue culture, and fibronectin (25 μg/ml), also protected spirochetes from complement-mediated lysis in contrast to BSK or fresh co-culture medium. Complement-mediated lysis may not be an effective mechanism in eradication of borrelia, and the chronicity of Lyme disease may be due to resistance ofB. burgdorferi variants to host immune defense mechanisms in the presence of host-derived tissues. Birkhäuser Verlag, 1996 Complement nationallicence co-culture nationallicence Lyme nationallicence Borrelia nationallicence pathogenicity nationallicence ESG nationallicence arthritis nationallicence fibronectin nationallicence Experientia Birkhäuser-Verlag 52/4(1996-04-01), 364-372 0014-4754 52:4<364 1996 52 18 https://doi.org/10.1007/BF01919542 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919542 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Güner E. Moda cad. 256/22, 81300 Kadiköy, Istanbul, (Turkey) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 364-372 0014-4754 52:4<364 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer